第一军医大学学报2000，Vol.20Issue(6)：499-503,5.
三种反义c-myc RNA稳定表达对血管平滑肌细胞生长、增殖的影响
Effects of three kinds of stable expressive antisense c-myc RNA on the growth and proliferation of vascular smooth muscle cell
摘要
Abstract
Ojective In order to understand the effects of continuous and stable expression of antisense genes on the growth and proliferation of the smooth muscle cells. Method Three combinant retrovial expression vectors, aM1, aM2 and aM3, which was respectively loaded with reverse fragment of c-myc exon 1, 2 and 3, were transferred into rat arterious smooth muscle cells and assayed 1 month after their stable expression. Result aM1 and aM2 could inhibit Myc expression (respectively reduced to 59.7%, 81.3%), and the expression of proliferat cellular nuclear antigen (PCNA) (respectively reduced to 52.4%, 51.9%). aM1 and aM2, especially the latter also inhibited cellular proliferative activity, aM2 slightly hindered cellular cycle and DNA synthesis too, while the effects of aM3 turned out to be contrary to that of aM1 and aM2. In addition, compared with transient expression, the effects of introduced genes were variable in target cells, and the changes brought about by the transfection process would reduce and disappear in a short time with the lowering of cellular compensation. Conclusions Blocking different regions of c-myc can achieve different effects and the blokade of the transcription activity regions usually produces enhanced growth inhibition effects. Moreover, target cells may have some 'correction mechanism ' against the introduced foreign genes.关键词
反义核酸/癌基因/增殖/血管平滑肌细胞/基因治疗Key words
antisense nucleic acid/oncogene/proliferation/vascular smooth muscle cell/gene therapy分类
生物科学引用本文复制引用
曾嵘,刘小蓉,李进,安靓,戴云..三种反义c-myc RNA稳定表达对血管平滑肌细胞生长、增殖的影响[J].第一军医大学学报,2000,20(6):499-503,5.基金项目
国家自然科学基金资助项目 （）
