癌症2001，Vol.20Issue(5)：502-504,3.
新鲜肿瘤标本活细胞与固定细胞的 DNA含量分析比较
DNA Content Comparison Analysis between Living Cells
and Fixed Cells in Fresh Tumor Samples
陶德定 1冷彦 1覃吉超 1余源 1龚建平1
作者信息
- 1. 华中科技大学同济医学院附属同济医院分子医学中心,
- 折叠
摘要
Abstract
Objective： This study was designed to establish a method of analyzing DNA content in fresh tumor using fresh cells. Methods： Clinical blood tumor samples were separated to obtain mononuclear cells by Ficoll-Hypaque, solid tumor tissues were made to be single cells by using mechanical method. The purified mononuclear cells and single cells suspensions were divided into two portions. One portion was fixed by ethanol and placed overnight, then measured DNA content by conventional flow cytometric method. The other portion was stained directly by PI （ propidium iodide) dissolved in PIPES (piperazine-Ν ,Ν '-bis[2-ethanesulfonic acid] disodium salt) buffer, then measured DNA content by flow cytometry, the DNA content results were compared with those of fixed cells. Results： There was no obvious difference in the CV（ coefficient of variation) of the peak of G0/G1 cells between fresh and fixed tumor cells from the same type tumor (t-test, P >0.05). Repetition test showed that the results of DNA content analysis using fresh cells were stable. Conclusion： Fresh tumor cells stained by PI dissolved in PIPES buffer could be used for the analysis of clinical tumor propagation and DNA ploidy.关键词
流式细胞术/肿瘤/新鲜细胞/DNA含量分类
生物科学引用本文复制引用
陶德定,冷彦,覃吉超,余源,龚建平..新鲜肿瘤标本活细胞与固定细胞的 DNA含量分析比较[J].癌症,2001,20(5):502-504,3.
