青岛大学医学院学报2001，Vol.37Issue(1)：50-52,3.
维生素C对葡萄糖测定的干扰及其作用机制
INTERFERENCE AND MECHANISM OF VITAMIN C ON THE DETERMINATION OF GLUCOSE
刘奉亭 1梁继伟 1柳忠旭 1庞瑞君 1冯健1
作者信息
摘要
Abstract
Objective To study the interference and mechanism of vitamin C on the determination of glucose. Methods Glucose concentrations were determined with GOD-PAP method and HK method. Results When a series of solutions containing same concentration of glucose but different concentrations of vitamin C was determined with GOD-PAP method, the determined glucose concentration decreased with the increase of vitamin C concentration in the solution, but vitamin C did not affect the determination of glucose with HK method. When glucose-free hydrogen peroxide solution was determined with GOD-PAP method, the apparent glucose concentration decreased with dilution of hydrogen peroxide; with constant hydrogen peroxide concentration in the solutions, the apparent glucose concentration decreased with increase of vitamin C concentration in the solutions. Conclusion Vitamin C interferes with the determination of glucose possibly through the reduction of the intermittent product hydrogen peroxide and the final product quinoneimine.关键词
葡萄糖/氧化还原酶类/维生素C/干扰分类
医药卫生引用本文复制引用
刘奉亭,梁继伟,柳忠旭,庞瑞君,冯健..维生素C对葡萄糖测定的干扰及其作用机制[J].青岛大学医学院学报,2001,37(1):50-52,3.
