中德临床肿瘤学杂志（英文版）2005，Vol.4Issue(1)：50-52,3.
双向分化肿瘤血管生成拟态分子机制初步观察
Pilot Study of Molecular Mechanism on Vasculogenic Mimicry in Bi-directional Differentiated Malignant Tumors
摘要
Abstract
To investigate the role of collagen Ⅳ and PAS positive substance secreted by tumor cells in vasculogenic mimicry (VM) and the effects of VM on tumor cells expressing VEGF. Methods: 158 cases of bi-direction differential malignant tumor specimens with paraffin-embedded were enrolled into our study and made tissue microarray which were dual-stained with CD31-PAS and stained with collagen Ⅳ.The difference of the areas and distribution with pattern surrounded by between CD31 and PAS positive respectively were identified via grid-counting, as well as the difference of VEGF expression with VE absent and present. Results: The basement membrane of VM was both PAS and collagen Ⅳ positive. VEGF expression in the bi-direction differential malignant tumor was higher VM-absent than VM-present and the difference was statistically significance in malignant melanoma and alveolar rhabdomyosarcoma (P＜0.05).Conclusion: PAS positive substance and collagen Ⅳ compose the wallof VE and VE could provide the oxygen and nutrition for tumor growth and progression.关键词
双向分化恶性肿瘤/血管生成拟态/重复染色/组织芯片Key words
bi-direction differential malignant tumor/vasculogenic mimicry/dual-staining/tissue microarray分类
医药卫生引用本文复制引用
孙保存,张诗武,赵秀兰,张丹芳,郝希山..双向分化肿瘤血管生成拟态分子机制初步观察[J].中德临床肿瘤学杂志（英文版）,2005,4(1):50-52,3.基金项目
This work was partially supported by a grant from the National Natural Science Foundation of China (No. 30370378) （No. 30370378）
