中国医学工程2003，Vol.11Issue(6)：50-53,4.
血管内皮生长因子在脊髓损伤后的表达及其意义
Expression of vasuclar endothelial growth factor(VEGF) after spinal cord injury(SCI)in rats
于利 1吕德成 1张岩2
作者信息
- 1. 大连医科大学附属一院,骨科,辽宁,大连,116011
- 2. 大连医科大学附属一院,病理科,辽宁,大连,116011
- 折叠
摘要
Abstract
Objective: To study the expression of vascular endothelial growth factor (VEGF) in spinal nerve cells after SCI in SD rats. Methods: 32 SD rats were divided at random into laminectomy and injury groups. The rats in injury group were injured in dorsal side of T10 spinal cord by Allen's stall. The rats were sacrificed at 8 h, 1 d, 2 d, 3 d, 1 w, 2 w and 3 w after surgery to sample spinal cord. Immunohistochemistry (SP) was performed to observe the distribution of VEGF in the spinal cord. Results: No VEGF immunoreactivity existed in normal spinal cords. Strong VEGF immunoreactivity was observed at 8 h after SCI. It peaked from 1 and 3 days and continued for 1 week. After 2 to 3 weeks immunoreactivity deereased significantly (P ＜ 0.01 ). VEGF positive rate of large motor neurons was higher than that of other kinds of nerve cells (P＜0.01). The positive rate of glial cells in dorsal white matter was higher than those in gray matter and ventral white matter (P＜0.01). Conclusion: The expression of VEGF in nerve cells in the injured spinal area was seen in the early stage of SCI in rats.关键词
脊髓损伤/血管内皮生长因子/基因表达Key words
spinal cord injury/vascular endothelial growth factor/gene expression分类
医药卫生引用本文复制引用
于利,吕德成,张岩..血管内皮生长因子在脊髓损伤后的表达及其意义[J].中国医学工程,2003,11(6):50-53,4.
