林业科学2007，Vol.43Issue(12)：50-55,6.
杉木第2代育种群体优良基因型的RAPD遗传变异
Molecular Characterization of Elite Genotypes within a Second-Generation Chinese Fir (Cunninghamia lanceolata) Breeding Population Using RAPD Markers
摘要
Abstract
RAPD markers were used to characterize the genetic diversity and relationships among 182 elite genotypes selected from second-generation trials as breeds for advanced generation breeding in Chinese Fir (Cunninghamia lanceolata). Twenty-nine 10-mer primers generated a total of 311 fragments,245 (78.8%) of which were polymorphic. Cluster analyses allowed for all 182 genotypes to be grouped into 11 clusters. AMOVA analyses showed that a high proportion (96.8%) of total genetic diversity resided within the field trials from which the elite genotypes were selected,whereas genetic diversity among field trials was low but significant (Φst=0.032,P<0.001). These results demonstrate that high levels of genetic diversity exist within the second-generation breeding population of Chinese Fir and hence suggest that there is good potential for genetic gains from further breeding.关键词
杉木/优良基因型/遗传变异/RAPDKey words
Chinese Fir(Cunninghamia lanceolata)/elite genotype/genetic diversity/RAPD分类
农业科技引用本文复制引用
李梅,施季森,李发根,甘四明..杉木第2代育种群体优良基因型的RAPD遗传变异[J].林业科学,2007,43(12):50-55,6.基金项目
Funded by a national key project of China (2002BA515B0101). （2002BA515B0101）
