山东科学2005，Vol.18Issue(3)：50-62,13.
Pseudomonas brassicacearum的数量分布差异及生物防治小麦全蚀病和油菜根腐病
Geographic differentiation of Pseudomonas brassicacearum populations and bio-control of take-all disease of wheat and Pythium root rot of canola
P. R. HARVEY 1R. A. WARREN 1W. ACHOUAK 2M. H. RYDER1
作者信息
- 1. 澳大利亚联邦科学院水土研究所, Glen Osmond, SA, 5064, Australia
- 2. CEA/Cadarache, DSV-DEVM, CNRS-CEA, F-13108 Saint-Paul-lez-Durance, France
- 折叠
摘要
Abstract
Strains representing populations of Pseudomonas brassicacearum from two contrasting South Australian wheat field soils were characterised by 3 PCR-based genotyping techniques and 3 phenotypic traits. Populations were highly diverse and significant levels of genotypic and bacterial-haplotype differentiation (GST and X2) were observed between the two geographical and two root-zone (i.e. rhizoplane and rhizosphere) populations(P<0.05).Three quarters of all strains had unique haplotypes and greater than 90% of these were specific to their population of origin. The average haplotype similarity among all strains was low (22%) and cluster analysis provided no evidence of relatedness on geographical origin or position in the root zone. These analyses indicate restrictions to inter-population gene flow and suggest that differentiation was due to random genetic drift and rhizoplane-based genotypic selection associated with the contrasting soils. One third of all strains tested positive for the 2,4-diacetylphloroglucinol biosynthesis gene(PhlD) and formed a related group (60% similarity).Four PhlD-positive strains(two wild types and their phenotypic variants) gave significant (P<0.05) bio-control of wheat take-all disease and Pythium damping-off of canola,indicating potential to control root disease complexes caused by these pathogens.关键词
Pseudomonas brassicacearum/遗传多样性/生物防治/全蚀病/PythiumKey words
Pseudomonas brassicacearum/genetic differentiation/biological control/take-all disease/Pythium分类
生物科学引用本文复制引用
P. R. HARVEY,R. A. WARREN,W. ACHOUAK,M. H. RYDER..Pseudomonas brassicacearum的数量分布差异及生物防治小麦全蚀病和油菜根腐病[J].山东科学,2005,18(3):50-62,13.
