动物学研究2007，Vol.28Issue(5)：507-510,4.
C57BL/6J小鼠在构建2型糖尿病模型中体重和非空腹血糖浓度的异常变化
Abnormal Change in Body Weight and Non-Fasting Blood Glucose Levels of Mouse Strain C57BL/6J in Generating Type 2 Diabetes Model
摘要
Abstract
The commercially available inbred obesity-prone C57BL/6J (B6) and outbred stock ICR mice (3-week old) purchased from a breeder of Beijing were weaned onto high-fat diet (HFD), HFD-3% fructose water (HFDF) and standard rodent chow, respectively. After exposure to the diets for six weeks, HFD and HFDF fed mice were injected intraperitoneaily with streptozotocin (STZ, 100mg/kg body weight) and kept on the same diet for next four weeks. Body weight was recorded weekly. Non-fasting blood glucose levels of HFD and HFDF fed mice were measured before and after STZ injections. The body weight of HFD-fed and HFDF-fed B6 mice were significantly lower than that of the control, but body weight of HFD-fed and HFDF-fed ICR mice were significantly higher than that of the control. After injection of STZ, blood glucose levels were above the stardardized criterion (11 mmol/L) for the diabetes mouse model in both HFD and HFDF fed ICR mice, but reverse in B6 mice. The type 2 diabetes model was generated successfully in ICR but not in B6 mice, regardless of whether fructose was supplied. The current results indicated that ICR mouse is still a useful and economical strain for HFD-induced/STZ-treated type 2 diabetes model, and that some variation may occur in the genetic composition among B6 mice bred by different breeders.关键词
C57BL/6J小鼠/ICR小鼠/高脂饲料/链脲佐菌素/肥胖/2型糖尿病Key words
C57BL/6J/ICR/High-fat diet/Streptozotocin/Obesity/Type 2 diabetes分类
生物科学引用本文复制引用
牛屹东,梁蜀龙,王新生..C57BL/6J小鼠在构建2型糖尿病模型中体重和非空腹血糖浓度的异常变化[J].动物学研究,2007,28(5):507-510,4.基金项目
北京大学人民医院研究与发展基金(P886)Supported by Peking University People's Hospital Research and Development Foundation (P886) （P886）
