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百合种质资源遗传多样性及亲缘关系的RAPD分析

童巧珍 周日宝 盛孝邦 刘湘丹 王朝晖 刘笑蓉 谭周进

农业现代化研究2009,Vol.30Issue(4):509-512,4.
农业现代化研究2009,Vol.30Issue(4):509-512,4.

百合种质资源遗传多样性及亲缘关系的RAPD分析

Genetic Diversity and Phylogenetic Relationship Analysis on Germplasm Resources of Lily by RAPD

童巧珍 1周日宝 2盛孝邦 2刘湘丹 1王朝晖 2刘笑蓉 2谭周进2

作者信息

  • 1. 湖南农业大学,湖南,长沙,410128
  • 2. 湖南中医药大学,湖南,长沙,410208
  • 折叠

摘要

Abstract

In this study, the genetic diversity and phylogenetic relationship among 16 genotypes of Lily collected from different ecological sources was analyzed by RAPD. Thirty-five available RAPD primers were screened from 120 random primers, and a total of 769 DNA bands were amplified, in which 628 bands were polymorphic, the percentage is 81.7%. Cluster analysis showed that the 16 Lily germplasms were divided into two groups at 0.9407. Corresponding to two genus in liliaceae: Lilium and Cardiocrinum, and that germplasms in Lilium were further divided into three different types at 0.5790: Liliam brownii F.E. Brown var.viridulum Baker, L. lancifotium Thunb and L. pumilum DC. Results suggested that the genetic diversity is very high among different Lily germplasms and the genetic distance among germplasms was relative to their geographic distance.

关键词

百合/种质资源/RAPD/聚类分析

Key words

lily/germplasm resources/RAPD/cluster analysis

分类

农业科技

引用本文复制引用

童巧珍,周日宝,盛孝邦,刘湘丹,王朝晖,刘笑蓉,谭周进..百合种质资源遗传多样性及亲缘关系的RAPD分析[J].农业现代化研究,2009,30(4):509-512,4.

基金项目

国家科技支撑项目"大宗道地中药材玉竹和百合规范化生产关键技术研究"(2006BA106A11-06) (2006BA106A11-06)

湖南省中医药管理局资助项目"湖南省药食两用百合种内居群的多样性研究及应用"(2008089). (2008089)

农业现代化研究

OA北大核心CHSSCDCSCDCSSCICSTPCD

1000-0275

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