安徽医科大学学报2001，Vol.36Issue(1)：5-11,7.
GM-CSF诱导人脐血CD34+造血干细胞上CXCR3的表达
CXCR3 expression on CD34+ hematopoietic progenitors from human cord blood induced by granulocyte-macrophage colony-stimulating factor
摘要
Abstract
Objective To investigate CXCR3 expression on CD34+ hematopoietic progenitors from human cord blood induced by granulocyte-macrophage colony-stimulating factor. Methods Using flow cytometry, real time quantitative reverse transcription (RT)-PCR assay, and its ligand γIP-10 and Mig-induced chemotaxis and adhesion assay. Results CXCR3 was expressed on GM-CSF-stimulated, but not freshly isolated, CD34+ hematopoietic progenitors from human cord blood. Freshly isolated CD34+ progenitors expressed low level CXCR3 mRNA, but this expression was highly up-regulated by GM-CSF.γIP-10 and Mig induced GM-CSF stimulated CD34+ progenitor chemotaxis via CXCR3 documented by the fact that anti-CXCR3 mAb blocks γIP-10 and Mig-induced CD34+ progenitor chemotaxis. These chemotactic attracted CD34+ progenitors were colony-forming unit-granulocyte macrophages.γIP-10 and Mig also induced GM-CSF-stimulated CD34+ progenitor adhesion via CXCR3, confirmed by the observation that anti-CXCR3 mAb blocks these functions of γIP-10 and Mig, but not of SDF-1α. γIP-10 and Mig-induced integrin (CD49a and CD49b) up-regulation plays crucial role in adhesion of GM-CSF-stimulated CD34+ progenitors. Conclusion CXCR3-γIP-10 and-Mig receptor-ligand pairs as well as the effects of GM-CSF on them may be especially important in cytokine/chemokine environment for the physiological and pathophysiological events of differentiation of CD34+ hematopoietic progenitors into lymphoid and myeloid stem cells, subsequently immune/inflammatory cells. These processes are including transmigration, relocation, differentiation and maturation of CD34+ hematopoietic progenitors.关键词
抗原,CD34/造血干细胞/胎血分类
医药卫生引用本文复制引用
..GM-CSF诱导人脐血CD34+造血干细胞上CXCR3的表达[J].安徽医科大学学报,2001,36(1):5-11,7.基金项目
中国国家自然科学基金 （）
