军医进修学院学报2001，Vol.22Issue(1)：51-53,3.
甲基化特异PCR方法对p16肿瘤抑制基因的研究
Study of p16 tumor suppressor gene with methylation-specific PCR
赵亚力 1李伯安 2谷志远 1郝好杰 1宋海静 1李琦1
作者信息
- 1. 解放军总医院基础所分子生物学研究室,
- 2. 解放军302医院免疫研究室,
- 折叠
摘要
Abstract
Objective: To investigate the p16 tumor suppressor gene promoter ringer methylation status in human lung cancer、 colonic cancer. Methods:To take advantage of methylation-specific polymerase chain reaction (MSP) as a main method, the status of 5CpG island of p16 gene. was analysed. Results:p16 gene from normal lung tissue show only unmethy]ation status. The human pulmonary giant cell carcinoma cell line (PLA801-D) amplified only with the methylation primers of p16 gene. The positive rate of p16 gene methylation status in lung cancer and colonic cancer was 60%(6/10) and 70%(7/10), respectively.Conclusion:The methylation status of p16 gene in tumor tissues is one of the innormal type of p16 gene. The detecting of methylation patterns will be possible to become a novel biomarker in detecting early-stage cancer. MSP should prove extraordinarily valuable clinically.关键词
p16肿瘤抑制基因/甲基化特异PCR方法/肺癌/结肠癌分类
医药卫生引用本文复制引用
赵亚力,李伯安,谷志远,郝好杰,宋海静,李琦..甲基化特异PCR方法对p16肿瘤抑制基因的研究[J].军医进修学院学报,2001,22(1):51-53,3.
