林产化学与工业2005，Vol.25Issue(3)：51-54,4.
前体饲喂对细胞培养生产紫杉烷的促进作用
ENHANCEMENT OF TAXANES PRODUCTION IN CELL SUSPENSION CULTURES OF TAXUS CHINENSIS BY PRECURSOR-FEEDING
摘要
Abstract
The effects of precursors on taxanes production in cell suspension cultures of Taxus chinensis(Pilg.)Rehd. were studied. Feeding of sodium pyruvate at 0.5 mmol/L enhanced the yields of taxol and 1β-hydroxy baccatin Ⅰat 336 % and 325.8 % of control, respectively. Taxuyunnanine C yield was greatly promoted in presence of 3-methyl-2-buten-1-ol at 2 mmol/L (318.6 % of control) and sodium benzoate at 0.4 mmol/L (345.2 % of control). Yield increase of 14β-(2-methyl-butyryl)oxo-2α,5α,10β-triacetoxytaxa-4(20), 11-diene was most pronounced in presence of sodium acetate (293 % of control at 1 mmol/L) and sodium pyruvate (310.4% of control at 0.5 mmol/L). The results showed that precursor-feeding at optimal concentration is advantageous to biosynthesis of taxanes.关键词
紫杉烷/细胞悬浮培养/前体Key words
taxanes/cell suspension culture/precursor分类
化学化工引用本文复制引用
周忠强,梅兴国..前体饲喂对细胞培养生产紫杉烷的促进作用[J].林产化学与工业,2005,25(3):51-54,4.基金项目
国家863资助项目(102-12-06-01) （102-12-06-01）
中南民族大学自然科学基金资助项目(YZZ02003) （YZZ02003）
