中国药科大学学报2007，Vol.38Issue(1)：51-54,4.
HPLC法同时测定丹参片中4种有效成分的含量
Simultaneous quantitative determination of four active components in Salvia miltiorrhiza tablets by HPLC
孙棣 1刘文英 1梁彩1
作者信息
- 1. 中国药科大学药物分析教研室,南京,210009
- 折叠
摘要
Abstract
Aim:To develop a method for rapid and sensitive simultaneous determination of the hydrophilic and lipophilic active components in Salvia miltiorrhiza tablets. Methods:RP-HPLC condition was established to separate danshensu,salvianolic acid B,cryptotanshinone and tanshinone ⅡA from each other on the Diamonsil C18(250 mm×4.6 mm ID,5 μm)column with the mobile phase consisted of methanol and 0.5% formic acid in gradient elution. Results:The four active components had good linearity. The average recoveries ranged between 96.63% to 100.3% with RSDs of less than 2%. Conclusion:The method appeared to be precise and accurate for the simultaneous determination of the four active components in Salvia miltiorrhiza Bunge tablets within 35 min.关键词
丹参素/丹酚酸B/隐丹参酮/丹参酮ⅡA/HPLCKey words
danshensu/salvianolic acid B/cryptotanshinone/tanshinone ⅡA/HPLC分类
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孙棣,刘文英,梁彩..HPLC法同时测定丹参片中4种有效成分的含量[J].中国药科大学学报,2007,38(1):51-54,4.
