作物学报2006，Vol.32Issue(4)：515-521,7.
籼粳杂种花粉不育基因的定位
Mapping of Loci for Pollen Sterility of Indica-Japonica Hybrids in Rice (Oryza sativa L.)
摘要
Abstract
To estimate QTL for the pollen sterility, an F2 population of 180 plants from an indica / japonica hybrid was tested with 110 molecular markers. By the genome wide screening, three QTLs were identified for the pollen sterility on chromosomes 3, 5, 7, respectively. In addition, a total of nine QTL for segregation distortion via male gametes were mapped on rice chromosomes, of which seven QTLs were suggested to be the same type of gamete abortion as found at ga14 and ga-11. The distortion data seemed to be more sensitive to detection of pollen abortion genes in the F1 hybrid than the morphological inspection of pollen. Two QTLs for the segregation distortion on chromosomes 5 and 6 indicated an abnormally high frequency of heterozygous genotype. At a locus indicated by qHPS-5, japonica-type homozygote showed a lower fertility level than the heterozygotes and indica-type homozygote. The molecular markers identified here would be useful in introducing a set of new and known sterility-neutral alleles to solve the pollen sterility problem in subspecific F1 hybrids. Further, the present results could be helpful to combine as many neutral alleles for distortion as possible on whole genome basis to stabilize the pollen fertility as a whole.关键词
水稻/数量性状基因座(QTL)/分子标记/花粉不育/杂种不育性Key words
Rice ( Oryza sativa L. )/Quantitative trait loci (QTL)/Molecular marker/Pollen sterility/Hybrid sterility分类
农业科技引用本文复制引用
陈静,江玲,王春明,池桥宏,翟虎渠,万建民..籼粳杂种花粉不育基因的定位[J].作物学报,2006,32(4):515-521,7.基金项目
Supported by National High-Tech Research and Development Program of China(863 Program) (2002AA2Z1001-06), High Technology Project of Jiangsu Province (BG2001301), and Program of Introducing International Super Agricultural Science and Technology (948 Program) (2004-Z24). （863 Program）
