南京大学学报（自然科学版）2003，Vol.39Issue(5)：516-521,6.
农杆菌介导的二氢黄酮醇3'5'羟化酶cDNA对非洲菊的转化及对其花色的影响
Agrobacterium-mediated Transfer of Flavonoid 3'5'-Hydroxylase cDNA to Gerbera hybrida Modifies Flower Colour
摘要
Abstract
A protocol for regeneration and genetic transformation has been established for gerbera(Gerbera hybrida ). High frequency regeneration of shoot buds from petioles was achieved withMurashige and Skoog's (MS) medium supplemented with 3 mg/L 6-benzylaminopurine (BA) and0.01 rng/L α-naphthalene acetic acid (NAA). Elongation of shoot buds and subsequent rooting wereobtained on MS medium with 0.1 nmg/L NAA. The Agrobacterium tumefaciens strain LBA 4404carrying a binary vector plasmid pEH subcloned with cDNA for flavonoid 3'5'-hydroxylase has beenused for transformation. The petiole explants from in vitro grown shoots were cocultivated with A.tumefaciens for 3 days. Shoot buds were produced on the regeneration medium containing kanamycin(25 mg/L) and carbenicillin (500 mg/L). The transgenic nature of the regenerated plants wasconfirmed by polymerase chain reaction and Southern hybridization analyses. Transgenic gerberaplants had obvious changes in flower colour and some alterations in the form of flowers and leaves.关键词
非洲菊,二氢黄酮醇3'5'羟化酶,花色,转化Key words
Gerbera hybrida, flavonoid 3' 5'-hydroxylase, flower colour, transformation分类
生物科学引用本文复制引用
郑丽屏,刘继梅,王玲仙,黄兴奇,钦佩..农杆菌介导的二氢黄酮醇3'5'羟化酶cDNA对非洲菊的转化及对其花色的影响[J].南京大学学报（自然科学版）,2003,39(5):516-521,6.基金项目
Natural Science Foundation of Yunnan (99C108M), Cooperative Project of Yunnan and Universities(B98-10K) （99C108M）
