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Inheritance Analysis and Identification of SSR Markers Linked to Late Blight Resistant Gene in Tomato

ZHU Hai-shan WU Tao ZHANG Zhen-xian

中国农业科学(英文版)2006,Vol.5Issue(7):517-521,5.
中国农业科学(英文版)2006,Vol.5Issue(7):517-521,5.

Inheritance Analysis and Identification of SSR Markers Linked to Late Blight Resistant Gene in Tomato

Inheritance Analysis and Identification of SSR Markers Linked to Late Blight Resistant Gene in Tomato

ZHU Hai-shan 1WU Tao 2ZHANG Zhen-xian2

作者信息

  • 1. Department of Vegetable, College of Agronomy and Bio-Technology, China Agricultural University, Beijing 100094, P.R. China
  • 2. Department of Horticulture, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, P.R. China
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摘要

Abstract

Late blight caused by Phytophthora infestans is the most serious disease of tomato production in China. Studies on the genetics of resistance and identification of molecular markers are very useful for breeding late blight resistant varieties.The objective of this paper was to study the inheritance of late blight resistance and identify simple sequence repeat (SSR)markers associated with resistance allele in tomato (Lycopersicon esculentum Mill). The results came from an F2 progeny of 241 plants derived from a cross between 5# inbred line that is susceptible to late blight and a resistant accession CLN2037E. The late blight responses of F2 plants were tested by artificially inoculation of detached-leaflets in plate and natural infection assayed under greenhouse conditions. Both methods showed that the resistance is dominant and inherited as monogenic trait. Genetic mapping and linkage analysis showed that the late blight resistance gene Ph-ROL was located on chromosome 9 with a genetic distance of 5.7 cM to the SSR marker TOM236.

关键词

disease resistance/late blight/Phytophthora infestans/SSR/tomato

Key words

disease resistance/late blight/Phytophthora infestans/SSR/tomato

分类

农业科技

引用本文复制引用

ZHU Hai-shan,WU Tao,ZHANG Zhen-xian..Inheritance Analysis and Identification of SSR Markers Linked to Late Blight Resistant Gene in Tomato[J].中国农业科学(英文版),2006,5(7):517-521,5.

基金项目

This study was sponsored by Key Project of 10th FiveYear Plan of Yunnan Provincial Government(200lNG21). (200lNG21)

中国农业科学(英文版)

2095-3119

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