广西植物2007，Vol.27Issue(3)：518-521,4.
淫羊藿嫩叶cDNA文库的构建
Construction of cDNA library in Epimedium brevicornum leaves
摘要
Abstract
The total RNA was extracted from young leaves of Epimedium brevicornum,and mRNA was purifled by oligo(dT)-cellulose column chromatography,which was reverse transcripted into cDNA using SMART(the Switch Mechanism At the 5'end of RNA Templates)technique.The resulting cDNA was digested by Sfi,the digested fragments were fractionated by CHROMA SPIN-400,and then ligated to λTriplEx2 vector,the mixture of ligation was finally packed into Lambda virons with packaging extracts.6×105 recombinants had been obtained.80 percent of products were over 500bp.20 clones were chosen randomly from the librarv and were screened cDNA inserts using PCR.The results showed that the cDNA library of young leaves of E.brevicornum had been constructed,and it could be used tO screening to isolate particular clones.关键词
淫羊藿/嫩叶/cDNA文库Key words
Epimedium brevicornum/young leaf/cDNA library分类
农业科技引用本文复制引用
钱宝英,黎云祥,杨子松,冯图..淫羊藿嫩叶cDNA文库的构建[J].广西植物,2007,27(3):518-521,4.基金项目
四川省杰出青年学科带头人培养计划(04ZQ026-047) （04ZQ026-047）
四川省科技厅应用基础项目(03JY029-021-22)[Supported bythe sichuanout-Standing Youth Academic Leader Cultivation Item(04ZQ026-047) （03JY029-021-22）
Science and Technology Department of Sichuan Province(03JY029-021.22)] （03JY029-021.22）
