中国临床康复2004，Vol.8Issue(24)：5189-5191,3.
饮食结构与链脲佐菌素对大白兔血脂组分和动脉粥样硬化形成的作用
Effects of dietary structure and streptozotocin on the blood lipid changes and atherosclerotic formation of New Zealand white rabbits
舒茂琴 1何作云 2何国祥 1宋治远 1王国超1
作者信息
- 1. 解放军第三军医大学西南医院心内科,重庆市,400038
- 2. 解放军第三军医大学新桥医院心内科,重庆,400038
- 折叠
摘要
Abstract
BACKGROUND: The apoE gene knock-out mice or apoE2-transgeneic mice are better models for studying hypertriglyceridemia and atherosclerosis (AS) ,but convenientand practical animal models are still lacking.OBJECTIVE: To probe into the effects of streptozotocin(STZ) and dietary structure on the blood lipid contents and AS lesions in the New Zealand White(NZW) rabbits, and create a suitable and useful animal model for the study of hypertriglyceridemia and AS.DESIGN: Randomized and controlled study.SETTING, MATERIALS and INTERVENTIONS: This experiment was carried out in the Central Laboratory of Southwest Hospital, Third Military Medical University of Chinese PLA. Male healthy NZW rabbits were selected and divided into 4 groups with the method of random sampling: standard rabbit small particles breeding group (SC group); high cholesterol breeding group(HC group); rabbit small particles breeding after STZ treatment group (STZ + SC group); high cholesterol breeding after STZ treatment group (STZ + HC group) . Effects of STZ and high cholesterol feed on blood lipid changes and AS lesion of NZW rabbits were compared and the relationship of AS lesion partial A scavenger receptor(SRA) expression and distribution of foam cells was analyzed.MAIN OUTCOME MEASURES: The concentrations of plasma lipoprotein and apolipoprotein and pharmacological changes of aortic tunica intima of rabbits were observed. Besides, relationship between the partial SRA expression of AS plaques and distribution of foam cells were analyzed.RESULTS: Triacylglycerol(TG) in plasm of rabbits in the STZ group [ (2.59 ± 0. 64) mmol/L] was mildly-medially higher than that of the SC group [(1.12±0. 34) mmol/L] (P ＜0.05); The high density lipoprotein cholesterol(HDL-C) decreased and large artery AS lesion occurred; In the STZ + HC group, plasm total cholesterol level of white rabbits[ (14.18 ±2.72) mmol/L]was similar to that of HC group[ (13.74±1.43) mmol/L], while plasm triacylglycerol level[ (3.84 ± 0.73) mmol/L] of the former group was much higher than that of the latter group[ (1.50 ± 0.35) mmol/L, P ＜0.05 ]; AS lesion of rabbits in STZ + HC group was most serious, and STZ alone could cause AS lesion, but its AS severity was a little milder than that of HC group. The blood lipid disorder caused by STZ was similar to that of most clinical patients with coronary heart disease, in whom most of the foam cells in the AS lesions came from monocyte-macrophage cells. Gene and protein expressions of SAR were the same as the histocyte distribution, its expression dose was in accordance with the degree of AS lesion.CONCLUSION: Animal models in hypertriglyceridemia and AS lesion treated with STZ and fed with normal feed are better for the study of hypertriglyceridemia and AS.关键词
链脲佐菌素/副作用/动脉粥样硬化/病因学/脂类/血液分类
医药卫生引用本文复制引用
舒茂琴,何作云,何国祥,宋治远,王国超..饮食结构与链脲佐菌素对大白兔血脂组分和动脉粥样硬化形成的作用[J].中国临床康复,2004,8(24):5189-5191,3.
