中德临床肿瘤学杂志（英文版）2008，Vol.7Issue(9)：523-526,4.
实时荧光定量RT-PCR检测鼻咽癌Survivin mRNA基因表达
Detection of Survivin mRNA in nasopharyngeal carcinoma by real-time fluorescence quantitative RT-PCR
摘要
Abstract
Objective:To establish the method of real time fluorescence quantitative RT-PCR for detecting the expression of Survivin mRNA in nasopharyngeal carcinoma (NPC) tissues.Methods:The total RNA was extracted from NPC cell line CNE-2 and tissues with Trizol and then been transcribed reversely to cDNA,a method of real time fluorescence quantitative RT-PCR for detecting the expression of Survivin mRNA in NPC tissues had been established,in which chronic nasopharyngitis patients' nasopharynx tissues treated as control group.Results:The expression of Survivin mRNA all could be detected either in CNE-2 cells,NPC tissues or in chronic nasopharyngitis patients' nasopharynx tissues,and there was higher the expression level of Survivin mRNA in NPC tissues than which in chronic nasopharyngitis patients' nasopharynx tissues,the difference was significant (P＜0.01).The expression of Survivin mRNA could be detected both in stage Ⅰ+Ⅱ and stage Ⅲ+Ⅳ NPC,and there was no significant difference in relative quantifications of gene expression between these two groups (P＞0.05).There was no relationship between Survivin mRNA expression and age and sex of NPC patients (P＞0.05).Conclusion:Real time fluorescence quantitative RT-PCR is a rapid,effective and high sensitive method for detecting the expression of Survivin mRNA in NPC tissues.The overexpression of Survivin mRNA may play some roles in pathogenesis of NPC.关键词
nasopharyngeal carcinoma (NPC)/real-time fluorescence quantitative RT-PCR/gene expression/apoptosis inhibitor SurvivinKey words
nasopharyngeal carcinoma (NPC)/real-time fluorescence quantitative RT-PCR/gene expression/apoptosis inhibitor Survivin分类
医药卫生引用本文复制引用
Shengmiao Fu,Junhong Cai,Zhihua Tu,Yutian Wang,Liqun Deng,Zhu Liang,Zhenqun Lin,Xuanju Gong..实时荧光定量RT-PCR检测鼻咽癌Survivin mRNA基因表达[J].中德临床肿瘤学杂志（英文版）,2008,7(9):523-526,4.基金项目
Supported by a grant from the National Natural Science Foundation of China (No.30460145). （No.30460145）
