畜牧兽医学报2001，Vol.32Issue(1)：52-57,6.
中国猪瘟兔化弱毒株（C-株）兔脾组织毒主要保护性抗原E2（gp55）基因序列分析
SEQUENCE ANALYSIS OF MAJOR PROTECTIVE ANTIGEN E2（gp55）
GENE OF CHINESE HOG CHOLERA LAPINISED VIRUS DIRECTLY
FROM RABBIT SPLEEN TISSUE（C-STRAIN）
韩雪清 1李红卫 2李小兵 1马军武 2涂长春 1胡弘博 2殷震 2蒋帅 3刘伯华 3赵启祖 1李健强 3李忠润 3谢庆阁3
作者信息
- 1. 中国农业科学院兰州兽医研究所,
- 2. 解放军农牧大学
- 3. 西北农业大学
- 折叠
摘要
Abstract
The major protective antigen E2 gene of Chinese Hog Cholera Lapinised Virus（C-strain）directly from rabbit spleen tissue，was amplified by reverse transcription （RT） and nested-polymerase chain reaction（N-PCR） and successfully cloned，it′s sequence was determined and compared with those of other virulent strains of HCV published natively or abroad．The result showed that，the nucleotide homologies of E2 gene of C-strain directly from rabbit spleen tissue with C strain（adapted to SK6 cell），C-strain（adapted to calf testicle cell，HCLV-C），HCV-SM strain（virulent strain Shimen），virulent strain Brescia（Netherlands）and virulent strain Alfort（Germany），were 98.87％，98.34％，94.58％，91.00％ and 80.78％ respectively．The amino acid homologies were respectively 98.95％，97.37％，94.22％，91.60％ and 89.23％．In three neutralized antigenic domains A、B、C of E2 gene compared with the strain described aboved and epidemic virulent strain，there was very little difference or even no difference between C-strain from rabbit spleen and C-strain adapted to cells，while the difference between C-strain form rabbit spleen and epidemic virulent strain，classic virulent strain in B、C domains was great．Morever compared with chinese classic virulent strain Shimen，there was great difference between our countrys′ virulent strains in 1980s and those in 1990s，this showed that the epidemic virulent strains of hog cholera virus have changed in our country．关键词
猪瘟/C-株/E2基因/序列分析分类
农业科技引用本文复制引用
韩雪清,李红卫,李小兵,马军武,涂长春,胡弘博,殷震,蒋帅,刘伯华,赵启祖,李健强,李忠润,谢庆阁..中国猪瘟兔化弱毒株（C-株）兔脾组织毒主要保护性抗原E2（gp55）基因序列分析[J].畜牧兽医学报,2001,32(1):52-57,6.
