中国病理生理杂志2001，Vol.17Issue(6)：527-530,4.
过氧亚硝基阴离子对气道上皮细胞的损伤作用
Airway epithelial injury induced by peroxynitrite
朱铁年 1赵瑞景 2凌亦凌 3谷振勇 3周君琳3
作者信息
- 1. 河北医科大学第四医院呼吸内科
- 2. 河北医科大学微生物与免疫学教研室
- 3. 河北医科大学病理生理教研室,
- 折叠
摘要
Abstract
AIM:To study the effect of ONOO- on the airway epithelial injury. METHODS: The mitochondrial respiration, the amount of lactate dedydrogenase (LDH) release into the cell culture medium, the levels of 8-hydroxy-2′-deoxyguanosine (8-OHdG), and the cellular apoptosis were examined after exposure of cultured rat tracheal epithelial (RTE) cells to ONOO-. RESULTS: Exposure of RTE cells to 0.25, 0.5 and 1 mmol/L ONOO- caused a dose-dependent suppression of the mitochondrial respiration . ONOO- also caused a dose-dependent increase in the percentage of LDH release. Exposure of RTE cells to ONOO- resulted in an increased generation of 8-OHdG in a dose-dependent manner. ONOO- caused an increase in apoptotic percentage in RTE cells in a time-dependent manner at different concentrations. CONCLUSION: ONOO- could cause necrosis and apoptosis in cultured RTE cells. Low concentration of ONOO- caused apoptosis in a time-dependent manner. Whereas exposure to high concentration of ONOO- resulted in cell necrosis, ONOO- caused a dose-dependent increase in the percentage of LDH release. Suppression of mitochondrial respiration and oxidative DNA damage by ONOO- may be the major cause of cellular injury induced by ONOO-.关键词
上皮/细胞/一氧化氮/凋亡/线粒体分类
医药卫生引用本文复制引用
朱铁年,赵瑞景,凌亦凌,谷振勇,周君琳..过氧亚硝基阴离子对气道上皮细胞的损伤作用[J].中国病理生理杂志,2001,17(6):527-530,4.
