首页|期刊导航|中国海洋湖沼学报（英文版）|Tyrosinase, a new innate humoral immune parameter in large yellow croaker (Pseudosciaena crocea R)
中国海洋湖沼学报（英文版）2009，Vol.27Issue(3)：527-535,9.DOI:10.1007/s00343-009-9178-z
Tyrosinase, a new innate humoral immune parameter in large yellow croaker (Pseudosciaena crocea R)
Tyrosinase, a new innate humoral immune parameter in large yellow croaker (Pseudosciaena crocea R)
摘要
Abstract
We evaluated the immune response to infection with a pathogen in large yellow croaker (Pseudosciaena crocea Richardson). The fish were given an intraperitoneal (i.p.) injection of Vibrio parahaemolyticus or sterile sea water (control). We collected blood sera from the fish 0.17, 1, 2, 4, 8, 12, or 16 d after injection (dpi). We measured tyrosinase activity and the concentrations of lysozyme, NOS, and antibodies. Serum tyrosinase activity was significantly higher at 0.17 and 4 dpi than in the control group, and peaked at 8 dpi. Lysozyme activity was significantly higher at 2 and 12 dpi than in the control group, but lower at 16 dpi. There is no statistical difference in the level of nitric oxides synthase (NOS) activity or antibodies between the control and injection groups. This is the first report of the tyrosinase activity in the serum of large yellow croaker. Our results indicate that tyrosinase plays an important role in the immediate immune defense against V. parahaemolyticus in large yellow croaker. Tyrosinase is a candidate parameter for investigation of fish innate immune defense.关键词
pseudosciaena crocea/ tyrosinase/ lysozyme/ nitric oxide synthase (NOS)/ natural antibodiesKey words
pseudosciaena crocea/ tyrosinase/ lysozyme/ nitric oxide synthase (NOS)/ natural antibodies引用本文复制引用
WANG Shuhong,WANG Yilei,ZHANG Ziping,XIE Fangjing,LIN Peng,TAI Zhengang..Tyrosinase, a new innate humoral immune parameter in large yellow croaker (Pseudosciaena crocea R)[J].中国海洋湖沼学报（英文版）,2009,27(3):527-535,9.基金项目
Supported by the Ministry of Education of China (No. 205079), the Education Department of Fujian Province (No. JA004229), the Sciences and Technology Department of Fujian Province (Nos. 2005N041, 2008N0121) and the Innovation Team Foundation of Jimei University (No. 2008A001) （No. 205079）
