南京大学学报（自然科学版）2009，Vol.45Issue(4)：529-536,8.
不同烷基酚诱导大鼠支持细胞膜通透性和细胞外基质分泌的对比研究
Comparative study of different alkylphenol induced effect on cell membrane permeability and extracellular matrix production of rat sertoli cells
摘要
Abstract
Alkylphenols (APs) as important environment contaminants have caused disruptive effects on male reproduction. The aim of present study was to compare different alkylphenol-induced effect on rat testicular sertoli cells. Alkylphenols, including p-cresol, 4-n-butylphenol and 4-n-nonylphenol, were treated to purify rat testicular sertoli cells, and their effects on cell membrane permeability and extracellular matrix production were studied and made comparison. The results revealed that alkylphenols increased membrane permeability (indicated as lactate dehydrogenase(LDH) leakage ratio) of sertoli cells in a dose-dependent manner within 12 h treatment, and the effect of butylphenol and nonylphenol was stronger than that of cresol which holds a shorter alkyl chain than the other two alkylphenols. When exposure time was extended to 24 h, LDH leakage reached a high-point at very low concentrations (5 ×10-8 mol/L for butylphenol and nonylphenoh 10-7 mol/L for cresol) and did not increase with dosage. In addition, exposure to alkylphenols induced laminin production in a dose-dependent manner, and the stimulative effect of butypbenol was more effective than that of cresol and nonylphenol.关键词
烷基酚/对比/lactate/dehydrogenase泄漏/层粘连蛋白/支持细胞Key words
alkylphenols/comparison/lactate dehydrogenase leakage/laminin/sertoli cells分类
生物科学引用本文复制引用
龚昳,袁春涛,吴江,屠志刚,韩晓冬..不同烷基酚诱导大鼠支持细胞膜通透性和细胞外基质分泌的对比研究[J].南京大学学报（自然科学版）,2009,45(4):529-536,8.基金项目
Foundation hem:National Natural Science]Foundation of China (20577019) （20577019）
