动物学研究2008，Vol.29Issue(5)：529-536,8.
交配行为对雄性棕色田鼠雌激素及雌激素β受体在相关脑区的影响
Changes of Estrogen in Serum and Estrogen Receptor β in the Relevant Brain Regions Following Mating Behavior of the Male Mandarin Vole Microtus mandarinus
摘要
Abstract
In order to investigate the estrogen and estrogen receptor β changes after mating behavior of male mandarin vole (Microtus mandarinus), the radioimmunoassay (RIA) and immunohistochemistry methods were used to investigate changes of the serum estrogen (E) concentrations, estrogen immunoreactive neurons (E-IRs) and estrogen receptor β immunoreactive neurons (ERβ-IRs) in the relevant brain regions following mating behavior. Fifteen sexually matured male voles were randomly divided into three groups and treated differently: (1) control group: voles were exposed to clean hard-wood shavings (n=5), (2) exposure group: voles were exposed to the soiled bedding for more than 24h on which estrous females had been placed (n=5), and (3) mating group: voles were placed with an estrous female for more than 24h (n=5). The results showed circulating serum E concentrations were significantly higher in the mating group than in the exposure group and the control group, and there were no significant difference between the exposure group and the control group. E-IRs and ERβ-IRs were detected in the following brain regions related to mating behavior: the arcuate nucleus (ARC), bed nucleus of the stria terminalis (BST), lateral septal nucleus (LS), medial amygdaioid nucleus (ME),medial preoptic area (MPO) and ventromedial hypothalamic nucleus (VMH). The results showed that there were significantly more E-IRs in the six brain regions in the mating group than in the control group and the exposure group, and there were no significant difference between the exposure group and the control group except for LS. There was no significant difference in ERβ-1Rs in the six brain regions among the three groups, and there were some lighter -stained ERβ-IRs in these brain regions. The results suggested that estrogen affect mating activity of male mandarin voles, hut ERβ might not play an important role in mating behavior of male mandarin voles. Instead, it might be through other receptors.关键词
棕色田鼠/雌激素/雌激素受体β/放射免疫分析/交配行为Key words
Mandarin voles (Microtus mandarinus)/Estrogen/Estrogen receptor β/Radioimmunoassay/Mating behavior分类
生物科学引用本文复制引用
何凤琴,张巨武,石靖,王波..交配行为对雄性棕色田鼠雌激素及雌激素β受体在相关脑区的影响[J].动物学研究,2008,29(5):529-536,8.基金项目
国家自然科学基金(30670273) （30670273）
陕西省自然科学基金项目(2008C269) （2008C269）
西安市科技局科技计划项目(YF07194) （YF07194）
西安文理学院专项科研资助项目(KY200520) （KY200520）
Fundation items: Natural Science Foundation of China (30670273) （30670273）
Natural Science Foundation of Shaanxi (2008C269) （2008C269）
Science and Technology Plan Project of Xi'an Bureau of Science and Technology (YF07194) （YF07194）
Special Science Research Fund for Xi'an University of Arts and Science(KY200520) （KY200520）
