中德临床肿瘤学杂志（英文版）2008，Vol.7Issue(9)：530-533,4.
肝细胞肝癌中拟态血管生成的三维细胞培养及组织学研究
Vasculogenic mimicry formation in hepatocellular carcinoma
Jing Zhao 1Chenyu Wang 1Aijun Yang 1Wei Liu 1Min Li1
作者信息
- 1. Department of Pathology,Lanzhou University,Lanzhou 730000,China
- 折叠
摘要
Abstract
Objective:To explore possibility of vasculogenic mimicry (VM) in hepatocellular carcinoma (HCC) by constructing tumor cell three-dimensional culture system and liver cancer tissues.Methods:Based on three-dimensional cell culture system developed by matrigel,liver cancer cell lines HepG2 were tested for evidence of VM.Fifteen HCC simples were collected.Potential formation of tumor channels and their characterization of network were observed by immunohistochemical and histological double staining of CD31 and PAS,or Ferritin and PAS.Results:Three-dimensional culture model of HCC cell line proved the liver cancer cells stretch out thin and long tubers at the second day,and the cells linked each other to form wreath and network structure at the seventh day.In fifteen HCC simples,endothelial cells were all stained by CD31,and tumor calls were all stained by Ferritin.The immunohistochemical and histological double staining also exhibited evidence of VM in seven simples of HCC,CD31-negative and Ferritin-positive tumor cells were observed to form tubal structure.Tumor cells were separated by PAS-positive matter like basement membrane from the tube.Red blood cells could be seen in the tube.In well-differentiated simples,VM was less than that in poorly differentiated ones,and several CD31-positive tumor cells could be observed in poorly differentiated simples.Conclusion:HepG2 cells have the capacity of self-metamorphose and vascularized trend.The tumor cells can obtain oxygen and nutrition through this structure.关键词
hepatocellular carcinoma (HCC)/vasculogenic mimicry (VM)/three-dimensional culture/immunohistochemical and histologicalKey words
hepatocellular carcinoma (HCC)/vasculogenic mimicry (VM)/three-dimensional culture/immunohistochemical and histological分类
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Jing Zhao,Chenyu Wang,Aijun Yang,Wei Liu,Min Li..肝细胞肝癌中拟态血管生成的三维细胞培养及组织学研究[J].中德临床肿瘤学杂志（英文版）,2008,7(9):530-533,4.
