中华医学杂志（英文版）2001，Vol.114Issue(5)：530-534,5.
一个在鼻咽癌中表达下调的新基因NAG-7的克隆及其特征分析
Molecular cloning and characterization of NAG-7: a novel gene downregulated in human nasopharyngeal carcinoma
摘要
Abstract
Objective To identify novel tumor suppressor genes at chromosome 3p24-26 in human nasopharyngeal carcinoma (NPC).Methods Twenty epithelial-derived expressed sequence tags (EST) were selected from chromosome 3p24-26. RT-PCR and Northern blot were used to detect the expression of the ESTs in NPC cell line, HNE-1, and primary cultures of normal nasopharyngeal epithelial cells. One EST, which was substantially downregulated in the HNE-1 cell line, was detected in 19 NPC biopsy samples, cDNA library screening was used to get its full sequence and the sequence of this novel gene was analyzed.Results A novel gene located at chromosome 3p25.3 was obtained and named NAG-7. It was downregulated in 26.3％ (5/19) of NPC biopsy samples. Its 1677 bp full length cDNA had a potential open reading frame predicting a 94 amino acid protein with a molecular weight of 11023.87 Dalton. Analysis of the NAG-7 gene showed that it was a transmembrane protein containing a protein kinase C phosphorylation site and a myristyl site. It has no significant homology to any reported genes in the database of GenBank. Conclusion NAG-7 is a novel gene downregulated in NPC, suggesting that it may be involved in the development of NPC.关键词
鼻咽肿瘤染色体3p基因克隆分类
医药卫生引用本文复制引用
谢奕,宾亮华,阳剑波,李忠花,余鹰,张小慧,曹莉,李桂源..一个在鼻咽癌中表达下调的新基因NAG-7的克隆及其特征分析[J].中华医学杂志（英文版）,2001,114(5):530-534,5.基金项目
This work was supported by a grant from the National 863 Project Foundation （）
