中国组织工程研究与临床康复2008，Vol.12Issue(27)：5343-5347,5.
构建与人肝细胞系L02相容的聚丙烯生物杂化界面
Establishment of L02 human hepatocyte compatible polypropylene interface
摘要
Abstract
BACKGROUND: Hepatocyte/polymer interface with good biocompatibility is the key factor in bioreactor design andconstruction, however, bioreactor used in the clinical practice currently is not an ideal one.OBJECTIVE: To establish human hepatocyte compatible polypropylene interface and to lay a foundation for establishingbioartificial liver reactor with polypropylene hollow fiber.DESIGN, TIME AND SETTING: The comparative observation, cell compatibility experiment was performed betweenFebruary and October 2003 at Shanghai Jiao Tong University, Shanghai, China.MATERIALS: Polypropylene Photochemical graft polymerization modification technique was used to graft hydrophilicacrylamide groups on the surface of polypropylene membrane by chemical bonds to form modified polypropylenemembrane.METHODS: L02 human hepatoeytes were seeded on polypropylene membrane, modified polypropylene membrane andpolystyrene membrane, and polystyrene membrane was used as normal control.MAIN OUTCOME MEASURES: Static water contact angle of polypropylene membrane before and after graftmodification; morphology, adherent rate and proliferation activity of L02 human hepatocytes on different material surfaces.RESULTS: Static water contact angle after polypropylene membrane graft modification was smaller than that before graftmodification (P < 0.05). The adherent rate of L02 human hepatocytes on the surface of modified polypropylene membranewas 0, and the proliferation activity of them, which grew as spherical aggregates, was markedly higher than that of cells onpolystyrene membrane and polypropylene membrane without graft modification.CONCLUSION: Grafting polyacrylamide on the surface of polypropylene can establish good interface of L02 humanhepatocytes/polypropylene and form hepatocyte spherical aggregates through simple static culture.关键词
肝细胞/人/聚丙烯类/球形聚集体/表面改性/生物材料分类
医药卫生引用本文复制引用
韩宝三,沈柏用,张瑞,高长有,马祖伟,王兆海,程东峰,彭承宏,李宏为..构建与人肝细胞系L02相容的聚丙烯生物杂化界面[J].中国组织工程研究与临床康复,2008,12(27):5343-5347,5.基金项目
国家高新技术研究发展计划一"八六三"项目(2007AA022487) （2007AA022487）
国家自然科学基金资助项目(30672043,30772105,20074031) （30672043,30772105,20074031）
上海市科委自然科学基金资助项目(07ZR14076) （07ZR14076）
the National High-tech Research and Development Plan of China (863 Project) （863 Project）
No. 2007AA02Z487 （）
the National Natural Science Foundation of China, No. 20074031, 30672043 and 30772105,20074031,the Natural Science Foundation of Shanghai Science and Technology Committee No.07ZR14076. （）
