中华医学杂志（英文版）2003，Vol.116Issue(1)：53-56,4.
Expression of hMSH2 gene and mutant p53 in sporadic digestive tract tumors
Expression of hMSH2 gene and mutant p53 in sporadic digestive tract tumors
康燕婕 1张振科 2王俊霞 3陈静 1彭勃 1康萍1
作者信息
- 1. Department of Gastroenterology, The Second Hospital of Baoding, Baoding 071051, China
- 2. Department of Gastroenterology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, China
- 3. Hebei Medical University, Shijiazhuang 050000, China
- 折叠
摘要
Abstract
Objective To investigate the role of mutated mismatch repair gene hMSH2 and mutant p53 gene in the carcinogenesis and development of sporadic digestive tract tumors. Methods hMSH2 gene in normal and tumor tissue of 30 digestive tract tumor specimens was examined using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) silver staining. The PCR product with an abnormal strand was sequenced directly. Mutant p53 protein in the tumor tissue was analyzed immunohistochemically. Results Six patients were identified as having mutated strands, three on hMSH2 exon 1 and three on hMSH2 exon 5. DNA sequencing revealed that all 6 patients had mutated basic groups that led to decrease in function of the hMSH2 protein. Forty percent (12/30) of patients were p53 positive. The frequency of mutated hMSH2 in p53 positive patients (41.7%) was significantly higher than in p53 negative patients (5.6%, P<0.05). Conclusion The mutation of hMSH2 plays an important role in the carcinogenesis and development of digestive tract tumors through stimulating p53 mutation.关键词
digestive tract tumor/mismatch repair gene hMSH2/mutant p53 geneKey words
digestive tract tumor/mismatch repair gene hMSH2/mutant p53 gene分类
医药卫生引用本文复制引用
康燕婕,张振科,王俊霞,陈静,彭勃,康萍..Expression of hMSH2 gene and mutant p53 in sporadic digestive tract tumors[J].中华医学杂志（英文版）,2003,116(1):53-56,4.
