福建农业大学学报(自然科学版)2001，Vol.30Issue(1)：53-58,6.
水稻条纹病毒外壳蛋白基因和病害特异性蛋白基因的克隆和序列分析
Cloning and sequence analysis of coat protein gene and disease-specific protein geneof Rice stripe virus
摘要
Abstract
Coat protein (CP) and disease-specific protein encoded by Rice stripe virus (RSV) are closely relatedto symptoms. We cloned and sequenced these two protein genes of YL isolate isolated from Yunnan Province ofChina. CP gene and SP gene were comprised of 966 nt and 534 nt, respectively. Compared with those isolatesthat have been published, we found that the sequence identities betweea C? gene of YL isolate and C isolate(isolated from Shandong Province of China), T, M isolates of Japan was obout 96.0% at the nucleotide level and97.0% at the amino acid level. However, C isolate was slightly closer to two Japan isolates than YL isolate according to sequence identities and the ways of base variation. There was 99.0% sequence identity between SPgene of YL isolate and CX isolate (also isolated from Yunnan Province of China) and only 94.0% among YL, Tand M isolates at the nucleotide level. These results indicated that variation of SP gene among different isolatesof RSV was closely relevant to their geographical location.关键词
水稻条纹病毒/外壳蛋白基因/病害特异性蛋白基因/序列分析分类
农业科技引用本文复制引用
林含新,魏太云,吴祖建,林奇英,谢联辉..水稻条纹病毒外壳蛋白基因和病害特异性蛋白基因的克隆和序列分析[J].福建农业大学学报(自然科学版),2001,30(1):53-58,6.基金项目
国家自然科学基金资助项目(39670489)及福建省自然科学基金资助项目(C97031). （39670489）
