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牙鲆脑中促性腺激素释放激素受体的克隆和鉴定

房保海 郑法新 孙修勤 曲凌云 张进兴

中国水产科学2006,Vol.13Issue(4):536-546,11.
中国水产科学2006,Vol.13Issue(4):536-546,11.

牙鲆脑中促性腺激素释放激素受体的克隆和鉴定

Cloning and tissue expression analysis of gonadotropin-releasing hormone receptor from the brain of Japanese flounder, Paralichthys olivaceus

房保海 1郑法新 2孙修勤 1曲凌云 2张进兴2

作者信息

  • 1. 中国海洋大学,生命科学与技术学院,山东,青岛,266003
  • 2. 国家海洋局第一海洋研究所,山东,青岛,266061
  • 折叠

摘要

Abstract

A full-length cDNA, encoding a GnRH receptor (GnRH-R), has been obtained from the brain of Japanese flounder (Paralichthys olivaceus). This cDNA encodes a protein of 415 amino acid residues exhibiting the typical arrangement of the Gprotein-coupled receptors with seven transmembrane domains. As already shown in other teleost fish, this protein possesses an intracellular carboxy-terminal tail, in contrast with its mammalian counterparts. In the case of Japanese flounder, this intracellular carboxy-terminal tail consists of 91 residues. Reverse transcription-polymerase chain reaction amplification products were found in the brain, the pituitary, the eyes and the ovary.

关键词

牙鲆/促性腺激素释放激素/促性腺激素释放激素受体

Key words

Paralichthys olivaceus/GnRH/GnRH receptor

分类

生物科学

引用本文复制引用

房保海,郑法新,孙修勤,曲凌云,张进兴..牙鲆脑中促性腺激素释放激素受体的克隆和鉴定[J].中国水产科学,2006,13(4):536-546,11.

基金项目

National Basic Research Program ("973" Program) of China (G1999012005). ("973" Program)

中国水产科学

OA北大核心CSCDCSTPCD

1005-8737

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