中国临床康复2004，Vol.8Issue(25)：5373-5375,3.
纳洛酮对血管性痴呆大鼠学习记忆能力和海马神经细胞内钙离子浓度的影响
Effects of naloxone on learning and memory ability and hippocampal [ Ca2+ ]i in rats with vascular dementia
摘要
Abstract
BACKGROUND: The alteration of opioid receptor during eerebral ischemia can induce dementia. Naloxone, opioid receptor antagonist, can modulate the alteration of opioid receptor during cerebral ischemia and thereby it may have preventive effects on vascular dementia(VD).OBJECTIVE: To investigate the preventive effects of naloxone on spatial learning and memory deficits in rats with VD for the discussion on its neural mechanism, mainly involving the [Ca2+ ]i in hippocampal neurons.DESIGN: A randomised controlled trial.SETTINGS and MATERIALS: VD model was established in 30 maleSprague Dawiey(SD) rats in a cleanness grade selected from the Experimental Animal-feeding Centre of the Medical College of Shantou University by permanent ligation in common carotid artery on both sides. Rats were randomly allocated into sham-operation group, model group and naloxone group.INTERVENTION: Naloxone was injected intraperitoneally and continuously for 7 days after model establishment in naloxone group and same amount of saline was injected intraperitoneally in the other two groups. Morris water maze(MWM) training method was used after 8 weeks.MAIN OUTCOME MEASURES: The escape latency(EL) of submergedplatform training, platform crossing times in spatial exploration experiment,the escape latency of visible platform training in rats of three groups, and the fluorescence pixel of the Ca2 + in the three groups under the laser scanning confocal microscope.RESULTS: The EL of MWM submerged platform training in rats of sham-operation group[(7.80 ±4.70) s] was significantly shortened compared with that of naloxone group and model group[ (8.10 ±2.93) s and (21.26 ± 17.41) s, respectively] ( F= 15. 028, P ＜0. 01), but there was no significance between sham-operation and naloxone group ( P ＞ 0. 05); The platform crossing times in spatial exploring experiments of the rats in sham-operation group[ (8.45 ± 1.19)/minute] significantly increased compared with that of naloxone and model group[ (8.00 ± 1.17)/minute and(4.44 ± 1.74)/minute, respectively] ( F = 23. 257, P ＜ 0. 01) but there was no significance between sham-operation group and naloxone group ( P ＞ 0. 05 ). There were no significant differences of the latencies of visible platform training among three groups ( P ＞ 0. 05) . The mean value of fluorescence pixel of the sham-operation group was 135.05 ± 29. 14; Naloxone group was 139.39 ± 30. 74; The model group was 484.05 ± 298.72, significanfiy higher than that of other groups( P ＜ 0. 01, F = 26. 363).CONCLUSION: Naloxone has significant preventive effects in spatial learning and memory deficits in VD rats. Its mechanism may be related to naloxone preventes the increase of the intracellular Ca2 + in hippocampal neurons.关键词
痴呆,血管性/药物疗法/纳洛酮/记忆/疾病模型,动物分类
医药卫生引用本文复制引用
郑先振,马光瑜..纳洛酮对血管性痴呆大鼠学习记忆能力和海马神经细胞内钙离子浓度的影响[J].中国临床康复,2004,8(25):5373-5375,3.基金项目
广东省自然科学基金项目(000825) （000825）
