中国临床康复2004，Vol.8Issue(25)：5394-5396,3.
血管性痴呆大鼠海马区的长时程增强变化
Changes of long-term potentiation of hippocampus in vascular dementia rats
摘要
Abstract
BACKGROUND: Long term potentiation(LTP) of CA1 region in the hippocampus of rats has been widely accepted as the cell model of learning and memory. However, there are few reports on its mechanism and pathological changes.OBJECTIVE: To observe the changes of LTP in CA1 region of rat' s hippocampus when blocking four blood vessels and explore the possible mechanism.DESIGN: Randomized case controlled study.SETTING, PARTICIPANTS and INTERVENTIONS: This experimentwas completed in the Experimental Animal Center of Field Operation Surgery Research Institute, Third Military Medical University of Chinese PLA. 60 Wistar rats were divided into control group and model group by random digits table method. Each group was divided into three subgroups by time phase which was 2 weeks, 4 weeks and 2 months, with each of 10 rats. Vascular dementia was produced in rats using a modification of Pulsinelli' s four-vessel occlusion model. Shuttle box system controlled by computer was used to test the learning and memory of rats. LTP of CA1 region induced by extracorporeal hippocampus slices was used to test the electricity changes of leaning and memory of rats. Ultrastructure of CA1 region of rat' s hippocampus was observed by transmission electron microscope.MAIN OUTCOME MEASURES: Active avoidance reaction(AAR); LTPdetection; ultramicro observation of CA1 region of hippocampus.RESULTS: Compared with control group, the AAR in model group declined more prominent than that of control group in the 2nd week( P ＜ 0.05) . It reduced much greater in the 4th week and 2nd months( P ＜ 0.01 ) . In the control group, the slices of hippocampus could obviously induce the LTP waveform while there was no LTP induced in all time phases in model groups. There were differences on the percentage changes of fEPSP slope before and after conditioned stimulus and between model groups and control groups( P ＜ 0.05) . However, there was no difference among different time phases. There were some chronically isehemic and anoxic changes could be seen in CA1 region of hippocampus among model groups, such as pyknosis of neuron, swelling chondriosome, reduced particles, increased electron density,demyelination of axon, formation of secondary lysosome, dilated vacuole of Golgi complex and vacuole of neuropil and so on.CONCLUSION: Detection of LTP in CA1 region of extracorporeal hippocampus slices can objectively reflect the short term memory impairment of rats which is a reliable cell model of learning and memory.关键词
长时程增强/痴呆,血管性/病理学/海马/病理学分类
医药卫生引用本文复制引用
姚国恩,王景周,陈曼娥,蒋晓江..血管性痴呆大鼠海马区的长时程增强变化[J].中国临床康复,2004,8(25):5394-5396,3.基金项目
重庆市科委资助项目(1999-2000) （1999-2000）
