中国临床康复2004，Vol.8Issue(25)：5414-5415,2.
神经生长因子对缺氧缺血性脑损伤细胞凋亡的影响
Impact of nerve growth factor on the apoptosis in hypoxic-ischemic brain damage
摘要
Abstract
BACKGROUND: Apoptosis is a mode of the death of neurocyte in hypoxic-ischemic brain damage. Study in vitro indicates that the deficiency of nerve growth factor(NGF)can induce apoptosis of neurocyte.OBJECTIVE: To discuss the relationship between hypoxic-ischemic brain damage(HIBD) and apoptosis for the research of the protective reaction of NGF against HIBD to provide a theoretical gist in the application of NGF against HIBD in neonates.DESIGN: A completely randomised controlled study.SETTINGS and MATERIALS: Forty of 7-day old SD rats with a body mass from 14 to 18 g in either gender was obtained from the experimental animal center of Nanjing Medical University, which were all SPF grade animals fed in a barrier environment.INTERVENTION: HIBD model was established in all 40 rats, which were randomly allocated into two groups: HIBD model control group ( n = 20) and NGF treatment group( n = 20), of which the animals were treated with NGF immediately after hypoxic-ischemia through abdominal cavity injection.MAIN OUTCOME MEASURES: The impact of NGF on the increase of body mass, mortality rate and the mass of the left and right brain were observed as well as the impact on apoptosis after hypoxic-ischemia by in situ nick end labelling(TUNEL) method.RESULTS: The increase of body mass in the rats of NGF treatment group was significantly higher than that of control group(P ＜ 0.01), while the mortality rate during the study of treatment group was significantly lower than that of control group. The brain mass of NGF group was similar to that of the healthy side(right side) of control group but the left side of treatment group [(0. 57±0.02) g] was significantly heavier than that of control group [ (0. 42 ±0. 01) g] (P ＜ 0. 01 ) . There was no significant difference of brain mass between left and right side in treatment group but there was significant difference of brain mass between left and right side in control group ( P ＜ 0.01) . The apoptotic features of apoptotic positive cells found in TUNEL staining were shrinking cell body, nuclear pyrosis, nuclear fragmentation and the formation apoptotic body. The number of positive cell in NGF group was significanfiy less than that of control group( P ＜ 0.01).CONCLUSION: NGF treatment can alleviate encephalatrophy and apoptosis after hypoxic-ischemia.关键词
神经生长因子/缺氧,脑/脑缺血/大鼠分类
医药卫生引用本文复制引用
陈吉庆,周艳,邵小松..神经生长因子对缺氧缺血性脑损伤细胞凋亡的影响[J].中国临床康复,2004,8(25):5414-5415,2.基金项目
南京医科大学科技发展基金资助项目(MA9917) （MA9917）
