中德临床肿瘤学杂志（英文版）2007，Vol.6Issue(6)：542-545,4.
PD98059对肝癌HepG2细胞Tec信号转导作用的初步研究
Effect of MEK1 inhibitor PD98059 on the signal transduction of Tec in HepG2 hepatoma cells
摘要
Abstract
Objective: To investigate the effect of MEK1 inhibitor PD98058 on Tec and ERK2 in HepG2 hepatoma cells.Methods: The expression of mRNA and protein of Tec and ERK2 in HepG2 cells was detected by immunocytochemistry assay. After various concentration of PD98059 treatment, the expression of Tec and ERK2 mRNA in HepG2 cells was detected by RT-PCR and Westem blotting. Results: Tec and ERK2 expressed highly in HepG2 cells. PD98059 obviously inhibited the expression of mRNA and protein of Tec and ERK2 in a dose-dependent manner, in which 40 μmol/L of PD98059 exhibited the strongest inhibiting effect. Conclusion: PD98058, as MEK1 inhibitor, can inhibit Tec, block the signal route of Ras/Raf/ERK and to impede the signal transduction in HepG2 cells. Tec may be the signal protein in the upper stream of Ras/Raf/ERK in hepatocarcinoma cells and is supposed to interact with the signal way of Ras/Raf/ERK.关键词
PD98059/ Tec/ ERK2/ hepatocarcinoma/ signal transductionKey words
PD98059/ Tec/ ERK2/ hepatocarcinoma/ signal transduction分类
医药卫生引用本文复制引用
..PD98059对肝癌HepG2细胞Tec信号转导作用的初步研究[J].中德临床肿瘤学杂志（英文版）,2007,6(6):542-545,4.基金项目
Supported by grants from the National Natural Science Foundation of China (No. 30370341, 30570410) and the Academic Foundation for Authors of National Excellent Doctoral Dissertation of China (No. 200261). （No. 30370341, 30570410）
