中国临床康复2004，Vol.8Issue(25)：5440-5441,2.
脑啡肽和多巴胺在实验性脑震荡大鼠脑组织中的表达及意义
Expressions of enkephalin and dopamine in brain tissue and their significance in cerebral coneussionai rats
摘要
Abstract
BACKGROUND: The mechanisms of cerebral concussion have remained poorly understood, and so do the expression profiles of enkephalin and dopamine and their significance in this injury.OBJECTIVE: To investigate the expressions of enkephalin and dopamine and explore their significance in rat cerebral concussion tissue.DESIGN: A randomized controlled trial.SETTING and MATERIALS: This study was conducted in the Academy of Military Medical Sciences of PLA. Totally 80 male Wistar rats of clean grade, provided by the Animal Center of Academy of Military Medical Sciences and given free access to water and food, were used for preparing animal models of cerebral concussion. The rats were randomly divided into 4 groups according to the different cerebral impact for model establishment using counterweights, namely the control group, 50, 100 and 200 g groups.INTERVENTIONS: The rats were sacrificed on the day 1, 3, 7, 14 and 30 following brain concussion, respectively, and the brain tissue were taken for examination of the expressions of enkephalin and dopamine by means of immunohistochemical staining.MAIN OUTCOME MEASURES: The manifestations, pathological changes, and the result of immunohistochemical staining on enkephalin and dopamine were observed.RESULTS: Typical features of cerebral concussion were seen in rats of the 100 g group. The main pathological changes .included cerebral vascular constriction and dilation, hemostasis and edema of the cerebral tissue and neuronal degeneration and necrosis. Enkephalin expression was enhanced on the day 1 after the injury, as seen in the cytoplasm of the endothelial cells in the cerebral cortex, hippocampus and cerebellum, and reached the peak level on day 7. From day 14, the expression of enkephalin decreased gradually, but still remained at higher-than-normal level until day 30. The expression of dopamine increased on day 7 and was seen in the cytoplasm of the endothelial cells and in the vascular wall in the cerebral cortex, hippocampus,thalamus and cerebellum, which, however, underwent no notable time-dependent changes.CONCLUSIONS: Cerebral concussion is characterized pathologically by blood circulatory disorder and degeneration and necrosis of the parenchymal cells. Enkephalin and dopamine may participate in the pathophysiological process of cerebral injury after cerebral concussion, and play important roles in blood vessel injury, regulation of blood-brain barrier and the degeneration and necrosis of the neural cells.关键词
脑震荡/脑啡肽类/多巴胺/免疫组织化学分类
医药卫生引用本文复制引用
彭瑞云,高亚兵,王德文,肖兴义,陈浩宇,杨瑞,刘杰,胡文华,马俊杰..脑啡肽和多巴胺在实验性脑震荡大鼠脑组织中的表达及意义[J].中国临床康复,2004,8(25):5440-5441,2.基金项目
全军"十五"医药卫生科研基金面上项目资助(01MB003) （01MB003）
