中国临床康复2004，Vol.8Issue(25)：5444-5445,2.
睡眠剥夺引起大鼠海马神经元凋亡及相关基因表达的变化
Neuronal apoptosis in hippocampus of rats caused by sleep deprivation and changes coordinated gene expression
章茜 1程江涛 1杨春 1王书春 1王一菱 2吴景兰 2王雨若1
作者信息
- 1. 郑州大学医学院,生理学教研室,河南省郑州市,450052
- 2. 郑州大学医学院,组织胚胎学教研室,河南省郑州市,450052
- 折叠
摘要
Abstract
BACKGROUND: To make sure whether sleep deprivation will cause cell degeneration and if its signal conduction is related to expressions of certain genes.OBJECTIVE: To explore the apoptosis of neutrons caused by sleep deprivation as well as the expressive changes of related genes.DESIGN: Randomized case controlled study.SETTING and MATERIALS: Experimental site: Physiological Laboratory of School of Medicine, Zhengzhou University. Adult SD rats provided by Experimental Animal Center of Henan Province which weighed about (200 ± 20) g were chosen of either sex.INTERVENTIONS: TUNEL dyeing method was used to observe the morphologic changes of neurons in hippocampus of rats caused by rapid eye movement sleep deprivation. The expressions of mRNA of bcl-2 and bax in hippocampus of rats were detected by in situ hybridization.MAIN OUTCOME MEASURES: The morphologic changes of neurons in hippocampus of rats caused by sleep deprivation; expressions of mRNA of bcl-2 and bax in hippocampus.RESULTS: The number of positive apoptosis neurons in CA1, CA3 regions of hippocampus in rats was increased after rapid eye movement sleep deprivation. The apoptosis rates of cells in CA1 to CA4 regions in hippocampus caused by paradoxical sleep deprivation were(6.60 ±2.24)% ,(1.69 ±0. 45)% , (6. 87 ± 1.32)% and (1.74 ±0. 98)% respectively.There was significant difference between the apoptosis rates of CA1, CA3 regions and CA2, CA4 regions( t =5.26 -6.95, P ＜0. 05). The value of positive signal expressions of bcl-2 mRNA was much stronger without different between four regions. The expression of bax mRNA was increased, there was difference between the expressions of CA1(211.12 ±59. 85), CA3(205.56 ±56.99)and CA2 ( 123.42 ± 22.80), CA4 ( 124. 21 ± 20.47 ) ( t = 3.20 - 4.36,P ＜ 0.05).CONCLUSION: Sleep deprivation can lead to neuronal apoptosis in hippocampus of rats. The expressive changes of mRNA of bcl-2 and bax may be the mechanism of neuron apoptosis.关键词
睡眠剥夺/海马/病理学/神经元/病理学/基因表达分类
医药卫生引用本文复制引用
章茜,程江涛,杨春,王书春,王一菱,吴景兰,王雨若..睡眠剥夺引起大鼠海马神经元凋亡及相关基因表达的变化[J].中国临床康复,2004,8(25):5444-5445,2.
