首页|期刊导航|中华医学杂志（英文版）|Transplantation of magnetically labeled mesenchymal stem cells improves cardiac function in a swine myocardial infarction model
Transplantation of magnetically labeled mesenchymal stem cells improves cardiac function in a swine myocardial infarction model
QI Chun-mei JU Sheng-hong MA Ming TANG Yao-liang MA Gen-shan LIU Nai-feng SHEN Cheng-xing CHEN Zhong LIU Xiao-jun HU Yao-peng ZHANG Xiao-li TENG Gao-jun
中华医学杂志（英文版）2008，Vol.121Issue(6)：544-550,7.
中华医学杂志（英文版）2008，Vol.121Issue(6)：544-550,7.
Transplantation of magnetically labeled mesenchymal stem cells improves cardiac function in a swine myocardial infarction model
Transplantation of magnetically labeled mesenchymal stem cells improves cardiac function in a swine myocardial infarction model
摘要
Abstract
Background Mesenchymal stem cells (MSCs) transplantation provides a new approach for myocardial repair.However,many important fundamental questions about MSCs transplantation remain unanswered.There is an urgent need to identify MSCs from the beating heart and analyze the efficacy of this new approach.This study aimed to localize the magnetically labeled MSCs(MR-MSCs)and monitor the restorative effects of MR-MSCs with magnetic resonance(MR) imaging.Methods Acute myocardial infarction(AMI)was created in swine by a balloon occlusion of the left anterior descending coronary artery.Cells were delivered via intracoronary infusion after myocardial infarction.Infarct size change and cardiac function were assessed with 3.0T MR scanner.The results were then confirmed by histological and western blot analysis.All statistical procedures were performed with Systat (SPSS version 12.01).Results A total of 26 swine were divided into four groups(sham-operated group,n=6;AMI group with PBS transplantation,n=6;labeled MSCs group,n=7;unlabeled MSCs group,n=7).MSCs,MR-MSCs(107 cells)or PBS were delivered by intracoronary injection after MI and serial cardiac MR imaging studies were performed at 0,4 and 8 weeks after transplantation.MR imaging demonstrated MI size decreased after MSCs transplantation in labeled and unlabeled groups,however,increases were seen in the AMI group at 8 weeks after MI.The left ventricular eiection fraction(LVEF) was slightly increased in the AMI group((41.87±2.45)%vs(39.04±2.80)%,P＞0.05),but significantly improved in the MR-MSCs group((56.85±1.29)%vs(40.67±2.00)%,P＜0.05)and unlabeled group((55.38±1.07)%vs(41.78±2.08)%,P＜0.05) at 8 weeks after treatment.MR-MSCs were further confirmed by Prussian blue and immunofluorescent staining.Western blot analvsis demonstrated that there was an increased expression of cardiomyocyte markers such as myosin heavy chain and troponin T in the MSCs treatment groups and the ratio of matrix metalloproteinase 2 to tissue inhibitor of metalloproteinase 1 decreased in the labeled group and unlabeled group compared with the AMI group and sham-operated group.Conclusion Transplanted MR-MSCs can regenerate new myocardium and prevent remolding In an MI model at 2-month follow-uD and represent a preferred method to better understand the mechanisms of stem cell therapy in future clinical studies.关键词
magnetic resonance imaging/contrast media/mesenchymal stem cell/myocardial infarction/ventricular functionKey words
magnetic resonance imaging/contrast media/mesenchymal stem cell/myocardial infarction/ventricular function分类
医药卫生引用本文复制引用
QI Chun-mei,JU Sheng-hong,MA Ming,TANG Yao-liang,MA Gen-shan,LIU Nai-feng,SHEN Cheng-xing,CHEN Zhong,LIU Xiao-jun,HU Yao-peng,ZHANG Xiao-li,TENG Gao-jun..Transplantation of magnetically labeled mesenchymal stem cells improves cardiac function in a swine myocardial infarction model[J].中华医学杂志（英文版）,2008,121(6):544-550,7.基金项目
This work was supported by the grants from the National Natural Science Foundation of China(No.30570743 and No.30670853)and Pre-investigation Item Of the Southeast University for Natural Science Foundation of China(No.XJ0590216). （No.30570743 and No.30670853）
