中国人兽共患病杂志2000，Vol.16Issue(6)：54-55,12,3.
问号赖型钩端螺旋体23kDa蛋白的免疫原性研究
STUDY ON THE IMMUNOGENICITY OF 23KDA ANTIGEN OF LEPTOSPIRA ERROGANS SEROVAR LAI
刘洪斌 1戴保民 2李胜富 2方之茂 2章涛3
作者信息
- 1. 宁波大学医学院宁波，315211
- 2. 华西医科大学钩研室
- 3. 福建医科大学分子医学教研室
- 折叠
摘要
Abstract
In order to test whether the 23kDa protein could be the candidate for new generation vaccines against leptospirosis for the developing world,the isolated 23kDa protein band was cut and injected twice into rabbits to raise anti-23kDa serum. What is more,the protection of E. coli lysate of pDL121 on guinea pigs was done. The results showed that the anti23kDa serum could recognize the sonicated antigen of L. interrogans serovar lai strain 017 and 23kDa protein expressed in pDL121 and the tilter of the antiserum were very high,approximately 1/12800. Injection of the E. coli lysate of pDL121 with Freund's adjuvant into guinea pigs resulted in some protection of the animal against the challenge with strain 017. This indicated that 23kDa had good imunogenicity and could serve as a candidate for protective antigen of L. interrogans.关键词
钩端螺旋体/免疫印迹/抗原Key words
Leptospira/Western Blotting/Antigen分类
医药卫生引用本文复制引用
刘洪斌,戴保民,李胜富,方之茂,章涛..问号赖型钩端螺旋体23kDa蛋白的免疫原性研究[J].中国人兽共患病杂志,2000,16(6):54-55,12,3.
