中国农业科学（英文版）2006，Vol.5Issue(7)：545-549,5.
Analysis of the Offspring Sex Ratio of Chicken by Using Molecular Sexing
Analysis of the Offspring Sex Ratio of Chicken by Using Molecular Sexing
摘要
Abstract
The overall sex ratio of offspring (dead embryos and hatch chicks) from all the fertilized eggs of 140 hens collected for30 days was studied using duplex PCR of certain fragments of sex chromosomes. Additional 894 dead embryos over a period of 21 days of incubation were also investigated to verify the sex ratio of the dead embryos. The sex of the early dead embryos was identified using this molecular sexing technique. The sex ratio of the hatch chicks and the total offspring of the hens investigated in this experiment did not differ from the expected sex ratio (i.e., 1:1). However, the number of female dead embryos was significantly more than that of males. The data indicated that the different physiologic function of males and females contributed to female-biased mortality during incubation. It was also found by further analysis that the sex ratios of the offspring of some hens were significantly biased to female or male over the period investigated, which suggested that the sex ratio of offspring might be influenced by the maternal condition to some degrees.关键词
chicken/offspring/sex ratio/sex identificationKey words
chicken/offspring/sex ratio/sex identification分类
农业科技引用本文复制引用
FENG Yan-ping,GONG Yan-zhang,Nabeel Ahmed Affara,PENG Xiu-li,YUAN Jin-feng,ZHAO Rui-xia,Mohammed Yusuf,Osman Jeffer,ZHANG Shu-jun..Analysis of the Offspring Sex Ratio of Chicken by Using Molecular Sexing[J].中国农业科学（英文版）,2006,5(7):545-549,5.基金项目
We acknowledge the financial assistance from the Key Project of Wuhan City (20022002061) and National Science and Technology Achievements Outreach Program of China (04EFN214200206). （20022002061）
