兰州大学学报（医学版）2007，Vol.33Issue(3)：54-58,5.
RP-HPLC同时测定苯巴比妥、苯妥英和卡马西平的血药浓度
Simultaneous determination of phenobarbitone, phenytoin and carbamazepine in human plasma by RP-HPLC
赵慧 1邱雯 1周素琴1
作者信息
- 1. 兰州大学第二医院,药剂科,甘肃,兰州,730030
- 折叠
摘要
Abstract
Objective To establish a method for the determination of phenobarbitone (PB), phenytoin(PHT) and carbamazepine (CBZ) in human plasma by reversed-phase HPLC at the same condition.Methods Agilent Zorbax 8 nm Extend-C18 (5 μm, 4.6 mm× 150 mm) was used as analytical column and mobile phase. The UV wavelength was 230 nm and flow rate was 0.8 mL/min. Results PB, PHT and CBZ were separated well. Resolving powers were all more than 1.5. The lowest detective concentrations of PB, PHT and CBZ were as following: 0.3802, 0.1823 and 0.1444μg/mL. The linear ranges were in the range of 0.4～60, 0.2～30, 0.2～30 μg/mL, and relative recovery rates were 97.04%, 96.52% and 97.09%respectively. The relative standard deviations of within-day and between-day were all less than 5.8%.Conclusion The method is simple, sensitive, convenient and reliable. It is suitable for the determination of phenobarbitone, phenytoin and carbamazepine in human plasma at clinic as normal test.关键词
反相高效液相色谱法/苯巴比妥/苯妥英/卡马西平/血药浓度Key words
RP-HPLC/ phenobarbitone/ phenytoin/ carbamazepine/ plasma concentration分类
化学化工引用本文复制引用
赵慧,邱雯,周素琴..RP-HPLC同时测定苯巴比妥、苯妥英和卡马西平的血药浓度[J].兰州大学学报（医学版）,2007,33(3):54-58,5.
