首页|期刊导航|中国农业科学（英文版）|Isolation, Identification of Differentially Expressed Sequence Tags in the Backfat Tissue from Meishan, large White and Meishan×Large White Cross Pigs
中国农业科学（英文版）2005，Vol.4Issue(1)：54-58,5.
Isolation, Identification of Differentially Expressed Sequence Tags in the Backfat Tissue from Meishan, large White and Meishan×Large White Cross Pigs
Isolation, Identification of Differentially Expressed Sequence Tags in the Backfat Tissue from Meishan, large White and Meishan×Large White Cross Pigs
摘要
Abstract
In order to detect the molecular mechanism of heterosis in pigs, the mRNA differential display technique was performed to investigate the differences of gene expression in the backfat tissue from Meishan,Large White and Meishan×Large White cross pigs.Nine 3'-end anchored primers in combination with ten 5'-end arbitrary primers were used to perform the differential display PCR and nearly 3 000 reproducible bands were examined .Fifteen expressed sepuence tags that were differentially expressed were isolated and then isdentifide through semi-quantitative RT-PCR.BLAST analysis revealed that the fifteen expressed sequence tags (ESTs) were ntt homologous to any of the known porcine genes or ESTs. These novel ESTs were then submitted to GenBank.关键词
Pig/mRNA differential display/Semi-quantitative RT-PCR/Expressed sequence tagKey words
Pig/mRNA differential display/Semi-quantitative RT-PCR/Expressed sequence tag引用本文复制引用
LIU Yong-gang,XIONG Yuan-zhu,DENG Chang-yan..Isolation, Identification of Differentially Expressed Sequence Tags in the Backfat Tissue from Meishan, large White and Meishan×Large White Cross Pigs[J].中国农业科学（英文版）,2005,4(1):54-58,5.基金项目
The authors wish to express thanks to Dr. Jiang Xunping for his encouragement and advice. This work was supported by grants from the National 973 Program of China (G2000016105). （G2000016105）
