邓医宇 1孙诚 1曾红科 1胡道松 2茹立强 2张苏明3
作者信息
- 1. 广东省人民医院急危重症医学部,广东省广州市,510080
- 2. 华中科技大学同济医学院神经物学教研室,湖北省武汉市,430030
- 3. 华中科技大学同济医学院附属同济医院神经内科,湖北省武汉市,430030
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摘要
Abstract
BACKGROUND: Ginkgo biloba extract(GBE) affects memory and cholinergic fibers in the hippocampus in VD rats, which is a hot topic of research at home and abroad at present and provides an important theoretical significance.OBJECTIVE: To study the therapeutic effect and its mechanism of GBE on vascular dementia.DESIGN: A randomized, controlled trial design.SETTING and MATERIALS: Research was completed at Neurobiology Department of Tongji Medical College, Huazhong University of Science and Technology. Male Wistar rats weighing 220- 280 g obtained from experimental animal center of Huazhong University of Science and Technology were employed.INTERVENTION: Forty-nine male Wistar rats were divided into two groups: sham-operation group ( n = 9) and VD model groups ( n = 40). Seven days after operation, the rats were tested for learning and memory ability by passive avoidance conditioned response. Thirty-six VD model rats which were unable to acquire the passive avoidance response were divided into four group randomly: model control group, GBE low dosage group, GBE moderate dosage group, GBE high dosage group, 9 rats for each group. The physiological saline 1 mL/100 g, GBE of 100, 150 and 200 mg/kg were applied in each group respectively by gastric infusion for 10 days. After which, the learning and memory ability of rats in every group were determined by the illuminated and dark chambers; and the density of hippocampal cholinergic fibers tissues in rats in all groups were observed by acetylcholinesterase staining.CA1 region.RESULTS: Compared with sham-operation group, in model group, during the learning test, the mistakes were remarkably increased and the cumulated stimulation time(CST) of electric shock were remarkably prolonged; During the memory test, the step through latency(STL) was remarkably shortened for the rats being shocked by electricity from an illuminated chamber to the dark chamber, the mistakes increased remarkably and CST remarkably prolonged ( P ＜ 0.01 ). Compared with model control group, in GBE moderate and high dosage groups, during the learning test, the escape latency(EL) for the rats escaping from the dark chamber to the illuminated one after the first electric shock was remarkably shortened and the mistakes in GBE low, moderate and high dosage groups remarkably reduced(P ＜ 0.01); During the memory test, in GBE low, moderate and high dosage groups, STL was remarkably prolonged, EL and CST shortened and the mistakes reduced. Under the fiberscope, it was observed that the density of cholinergic fibers in the hippocampus CA1 region was remarkably sparse and the absorbance decreased ( P ＜ 0.01) in the model group, compared with the shain-operated groups.The density of cholinergic fibers in the hippocampus CA1 region was remarkably dense and the absorbance was increased( P ＜ 0. 01 ) in GBE low,moderate and high dosage group, compared with the model group, indicating that GBE may increase the content of cholinergic fibers in the hippocampus CA1 region in VD rats.CONCLUSION: GBE achieves significant therapeutic effect on VD in rats and its mechanism is probably related to the improvement of the density of cholinargic fibers in the hippocampus.关键词
痴呆,血管性/药物疗法/海马/胆碱能纤维/银杏/化学/植物提取物/药理学分类
医药卫生
