中药材2009，Vol.32Issue(4)：548-556,9.
灯盏花素改善大鼠局灶性脑缺血后空间学习记忆功能的研究
Breviscapine Improves Functions of Spatial Learning and Memory of Focal Cerebral Ischemia Rats
摘要
Abstract
Objective:To investigate the effects of breviscapine on the functions of spatial learning and memory of focal cerebral ischemia rats. Methods:Rots withl the left middle cerebral artery occluded were made by an intraluminal filament. Then breviscapine (20 mg/kg,40 mg/kg) in experimental group and 10% glucose in control group were administered intraperitoneally once a day for 2 weeks,and the Morris water maze tasks were carried out for 5 days. Results:Compared with sham-operation group,the animals of ischemia-control group exhibited seriously impaired spatial learning and memory in both place navigation test and spatial probe test. In the place navigation test,the mean value of escape latency in breviscapine group was significantly shorter than that in ischemia control group (P＜0.01 for lower-dose and P＜0.05 for higher-dose breviscapine group, respectively). In the spatial probe test,compared with sham-operation (P＜0.01) and breviscapine group (P＜0.01),the rats of ischemia-control group spent more time in the no-former platform quadrant,and showed reduced frequency of crossing former platform site significantly. The numbers of neurons with Nissl staining and choline acetyltransferase (ChAT) immunopositive neurons in ipssilateral cortex in the breviscapine group were significantly more than those in the ischemia-control group (P＜0.01). In hippocampus,the numbers of neurons with Nissl staining and ChAT immunopositive neurons in the stratum pyramidale of CA area were similar among the groups. Conclusion:These results indicate that breviscapine can improve the functions of spatial learning and memory of focal cerebral ischemia rats and the protection against the loss of ChAT immunopositive neuron in new cortex may be involved in its mechanisms.关键词
灯盏花素/学习记忆/脑缺血/乙酰胆碱转移酶Key words
Breviscapine/Learning and memory/Cerebral ischemia/Choline acetyltransferase分类
医药卫生引用本文复制引用
刘建新,刘勇,陈新林,赵建军,宋土生,钱亦华..灯盏花素改善大鼠局灶性脑缺血后空间学习记忆功能的研究[J].中药材,2009,32(4):548-556,9.基金项目
国家自然科学基金项目(30170300、30500575) （30170300、30500575）
