中国组织工程研究与临床康复2009，Vol.13Issue(28)：5527-5533,7.DOI:10.3969/j.issn.1673-8225.2009.28.027
颈动脉内膜切除后基质金属蛋白酶9动态表达对早期血管再狭窄形成的意义
Role of matrix metalloproteinase-9 dynamic expression in the development of early vascular restenosis after carotid endarterectomy
梁春阳 1戴宜武 1沈春森 1魏群 1李运军 1罗永春1
作者信息
- 1. 解放军北京军区总医院神经外科,北京市,100700
- 折叠
摘要
Abstract
BACKGROUND: Vascular restenosis alter carotid endarteractomy (CEA) is an important factor affecting curative affect ofoperation.OBJECTIVE: To explore the role of matrix metalloproteinase-9 (MMP-9) mRNA dynamic expression in the development of early vascular restenosis after carotid endarterectomy.DESIGN, TIME AND SETTING: A random grouping contrast observation was completed in the General Hospital of Beijing Military Area Command of Chinese PLA from February 2006 to December 2007. MATERIALS: Forty-one healthy male New Zealand rabbit, weighing about 3.0 kg, with 36 ones used for preparing carotid atherosclerotic stenosis (CASS) models. experimental group, each 6 of the CASS rabbit models (n =36) were selected at the time points of hour 4, day 1, 3, 7, 30, and 90 following CEA respectively. Then they were fixed with 40 g/L polyoxymethylene perfusion and stained with hematoxylin-eosin to observe their morphologic changes.MAIN OUTCOME MEASURES: The expression changes of MMP-9 mRNA were observed dudng the development of early vascular restenosis by the quantitative real-time polymerase chain reaction technique preoperatively as well as at day 1, 3 and 7 following CEA.RESULTS: Several stages could be seen in the reparative process of neointima after CEA, including the thrombosis, the inflammatory reaction, the repair of endothelium, the proliferation of vascular smooth muscle call, the formation and accumulation of extracellular matrix. MMP-9 mRNA was expressed since day 1, reached a peak at day 3 and then decreased significantly at day 7 postoperatively.CONCLUSION: MMP-9 plays an important role in the proliferation, migration and reconstruction of vascular smooth muscle calls, the mediated reconstruction of local blood vessels, as well as the development of vascular restenosis.关键词
颈动脉内膜切除术/再狭窄/基质金属蛋白酶9分类
医药卫生引用本文复制引用
梁春阳,戴宜武,沈春森,魏群,李运军,罗永春..颈动脉内膜切除后基质金属蛋白酶9动态表达对早期血管再狭窄形成的意义[J].中国组织工程研究与临床康复,2009,13(28):5527-5533,7.
