中国病理生理杂志2001，Vol.17Issue(6)：553-555,3.
圆头精子的冷冻保存
Cryopreservation of round-headed human sperm
朱伟杰 1梁蔚波2
作者信息
- 1. Center for Reproductive Immunology Research
- 2. 暨南大学生殖免疫研究中心
- 折叠
摘要
Abstract
AIM: To investigate the cryopreservation of round-headed (globozoospermia) human sperm. METHODS: Round-headed sperm samples (n=13) were obtained from 6 infertile men. Yolk-free cryoprotective medium and the two-step freezing were used in cryopreserving samples. Frozen sperm functions were examined by using motility evaluation, sperm head-tail membrane integrity test, and survival time test. RESULTS: The results showed that sperm motility, membrane integrity rates, survival time in vitro significantly lowered after thawing (n=13, P＜0.01). No acrosomal proteinase activity was detected in both fresh and frozen sperm groups (n=5). There were no significant difference in post-thawing motility between yolk-free cryoprotective medium and yolk medium, and between two-step freezing and multiple-step freezing (n=7, P＞0.05). CONCLUSION: Human round-headed sperm could undergo the freezing-thawing process. However, cryo-survival rates were low. Frozen sperm functions were significantly decreased. Yolk-free cryoprotective medium and two-step freezing could be used in the cryopreservation of round-headed sperm.关键词
低温保存/精子分类
医药卫生引用本文复制引用
朱伟杰,梁蔚波..圆头精子的冷冻保存[J].中国病理生理杂志,2001,17(6):553-555,3.
