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中华医学杂志（英文版）2003，Vol.116Issue(4)：554-557,4.
Effect of high glucose, angiotensin Ⅱ and receptor antagonist Losartan on the expression of connective tissue growth factor in cultured mesangial cells
Effect of high glucose, angiotensin Ⅱ and receptor antagonist Losartan on the expression of connective tissue growth factor in cultured mesangial cells
摘要
Abstract
Objective To observe the effect of high glucose, angiotensin Ⅱ (AngⅡ) and Losartan on the expression of connective tissue growth factor (CTGF) mRNA in cultured mesangial cells (MCs). Methods MCs of SD rats were isolated and cultured. High glucose (30 mmol/L) and AngⅡ (10-9, 10 7, and 10-5 mol/L) were added to the medium for 72 hours to observe the influence on CTGF mRNA expression. Losartan of 10-5 mol/L and AngⅡ of 10-5 mol/L were added to the medium to observe the effects of Losartan on CTGF mRNA expression stimulated by AngⅡ. The expressions of CTGF mRNA were detected by reverse transcriptase polymerase chain reaction (RT-PCR).Results RT-PCR showed that high glucose and AngⅡ up-regulated the expression of CTGF mRNA, and AngⅡ stimulated the expression in a dose-dependent manner. Expression of CTGF mRNA induced by AngⅡwas partially suppressed by 10-5mol/L Losartan (P<0.05).Conclusions High glucose and AngⅡ can enhance the expression of CTGF mRNA and thus be involved in the process of renal fibrosis. Losartan can have a partial fibrogenesis-inhibiting effect, with implications for the treatment of renal fibrosis.关键词
high glucose/angiotensin Ⅱ/Losartan/connective tissue growth factor *mesangial cells/renal fibrosisKey words
high glucose/angiotensin Ⅱ/Losartan/connective tissue growth factor *mesangial cells/renal fibrosis分类
医药卫生引用本文复制引用
黄颂敏,刘芳,沙朝晖,付平,杨一帆,徐勇,周海燕..Effect of high glucose, angiotensin Ⅱ and receptor antagonist Losartan on the expression of connective tissue growth factor in cultured mesangial cells[J].中华医学杂志（英文版）,2003,116(4):554-557,4.基金项目
The study was supported by the grants from the National Nature Science Foundation of China (No.39870297). （No.39870297）
