华西医科大学学报2001，Vol.32Issue(1)：55-58,4.
哮喘气道炎症中嗜酸性粒细胞凋亡与 IL-5、IL-10关系的研究
A Kinetic Study on the Relationship Between of IL-5, IL-10 and Eosinophil Apoptosis in Asthmatic Airway Inflammation
摘要
Abstract
Objective To observe time-course profile of eosi nophilapoptosis, in murine asthma models in airway explicate its implication fo r asthmatic inflammation remission, and investigate the role of IL-5 and IL -10 in modulating cell apoptosis. Methods An animal model of a sthma was establi shed by OVA sensitizing-challenging BALB/c mice. Histologic study of lung tissu e was made with the use of electronmicroscope. At seven points (0h , 8h, 24h, 48h, 96h, Day 7 and Day 14) in the time course after challenge,bronchoalveolar lavage (BAL ) was performed obtain BAL cells and recover fluid. Levels of IL-5 and IL-10 were det ec ted by ELISA. Cell apoptosis was assayed by PI stain on a flow cytometer. Results After challenge, apoptosis of eosinophils, lymphocytes,endoth elial cells could be seen under the electronmicroscope. IL-5 in BALF elevated significantly where a s no significant difference was found between the levels of IL-10 in BALF befor e and after challenge. Eosinophil apoptosis was identified and a two-phase eleva t ion of apoptosis percentages was observed,although no significant difference was found between average apoptosis in asthmatic group and that in control. Data an alyses revealed a negative correlation between apoptosis percentages and the lev els of IL-5, and a closer correlation between the percentages and IL-5/IL-10 rat io. Conclusion IL-5 and IL-10 could up- and down- regulate as thmatic airway inflammation, respectively. EOS underwent apoptosis in situ and t his was modulated by IL-5 and IL-10 interaction.关键词
哮喘/嗜酸性细胞/细胞因子/凋亡分类
医药卫生引用本文复制引用
刘春涛,王曾礼,冯玉麟,雷松..哮喘气道炎症中嗜酸性粒细胞凋亡与 IL-5、IL-10关系的研究[J].华西医科大学学报,2001,32(1):55-58,4.基金项目
国家自然科学基金资助项目 （）
