大豆科学2008，Vol.27Issue(4)：556-563,8.
大豆种子发育过程中差异表达蛋白的蛋白质组分析
Proteomic Analysis of Differentially Expressed Proteins in Developmental Soybean [ Glycine max( L. )Merr. ] Seed
摘要
Abstract
A proteomic approach was applied in the study on the changes in proteins that occur during seed development in soybean [ Glycine max( L. )Merr. ]cv N2899. Protein expression at different developmental stages (15,20,30,40,50 DAF and mature seed)were compared using PDQuest software (Bio-Rad)after two-dimensional gel electrophoresis. About 337 low-salt extractable protein spots were resolved in gels. It was found that some proteins were present throughout the whole developmental stages,while others were associated with early seed filling or seed maturation. Among total spots 18 of 30 highly differentially expressed proteins were identified by MALDI-TOF-MS, querying peptide mass fingerprinting data in NCBInr database by Profound(http://www. prowl. rockefeller. edu). It was found that these identified proteins are mainly involved in seed maturation( e. g. conglycinin), stress tolerance ( e. g. ascorbate peroxidase), cell division ( e. g. Skpl ), and proteins transportation( e. g. calreticulin). These results indicate that the seed development process is complex,and the iden tified genes would provide a basis for molecular elucidation of the temporal effects on protein accumulation in soybean seed.关键词
大豆/种子/MALDI-TOF-MS/蛋白质组Key words
Clycine max ( L. ) Merr/MALDI-TOF-MS/Proteomics/Seeds分类
农业科技引用本文复制引用
郑蕊,徐晓燕,李春梅,盖钧镒,喻德跃..大豆种子发育过程中差异表达蛋白的蛋白质组分析[J].大豆科学,2008,27(4):556-563,8.基金项目
National Natural Science Foundation of China ( No. 30771362) （ No. 30771362）
National 863 Project ( No. 2006AA10Z1C1) （ No. 2006AA10Z1C1）
111 Project of Ministry of Education (B08025). （B08025）
