白求恩医科大学学报2000,Vol.26Issue(6):559-561,3.
人WAF1基因表达质粒的构建
Construction of expression plasmid in human WAF1 gene
摘要
Abstract
Objective :Humna WAF1 gene was cloned,and WAF1-pcDNA3 recombinant plasmid was con-structed. Methods :Human WAF1 gene was amplified by RT-PCR from Hela cell,then cloned into pcDNA3vector. Results :Human WAF1-pcDNA3 recombinant plasmid was constructed,and DNA direct sequencingindicated that was correct. Conclusion:Our study laid the foundation for studying WAF1 gene expressionand it's biological effects.关键词
WAF1/基因克隆/直接测序Key words
WAF1/gene cloning/direct sequancing分类
生物科学引用本文复制引用
侯治富,王维忠,王华,吴晓冬,卜丽莎,王丽颖..人WAF1基因表达质粒的构建[J].白求恩医科大学学报,2000,26(6):559-561,3.基金项目
吉林省科技发展计划项目(970567-1) (970567-1)