肿瘤防治研究2009，Vol.36Issue(7)：560-562,3.DOI:10.3971/j.issn.1000-8578.2009.07.006
葡萄糖和胰岛素对Colon26肿瘤细胞分泌TGF-β1的影响
Effect of Glucose and Insulin on Secretion of TGF-β1 in Colon26 Cells
摘要
Abstract
Objective To observe the effect of different concentrations of glucose and insulin on the secre-tion of TGF-β1 in Colon26 tumor cells. Methods According to the concentrations of glucose and insulin in culture medium, cells were divided into 12 groups. After glucose and insulin were added into different groups, cells were cultured for 48h. In all experiments TGF-β1 concentration in the supernatant were de-tected by ELISA. Results (1) In group D, the level of TGF-β1 was higher significantly than group A(P <0.01). However no differences between group B and A, C and group A were detected(P>0.05). (2) In group E, F and G, compared with A, there was no statistical difference about the secretion of TGF-β1 in Color26 tumor cells (P>0.05). (3) In group L, M and N, the levels of TGF-β1 were higher signifi-cantly than A(P<0.01). However no differences between group H and A, K and A was detected(P> 0.05). Conclusion (1)High concentration of glucose may up-regulate the secretion of TGF-β1 in Color26 tumor cells. (2) Different concentrations of insulin have no effect on the secretion of TGF-β1 in these cells.关键词
葡萄糖/胰岛素/TGF-β1/糖尿病/结直肠癌Key words
Glucose/Insulin/TGF-β1/Diabetes mellitus/Colorectal cancer分类
医药卫生引用本文复制引用
周慧敏,宋小天,张素敏,刘凤娟,王润田..葡萄糖和胰岛素对Colon26肿瘤细胞分泌TGF-β1的影响[J].肿瘤防治研究,2009,36(7):560-562,3.基金项目
河北省重大疾病专项资助项目(07276103D) （07276103D）
